生长方案:	Seeds were sown singly in 50 mL conical centrifuge tubes modified with a drain hole and filled with LC1 soilless medium supplemented with 7.5g/L 20-20-20 Osmocote fertilizer. Plants were grown in a growth chamber providing 575 mM m-2s-1 of PAR at a R:FR of 13.30 from t5 fluorescent lamps with a photoperiod of 16/8 light dark and 32/22oC day night temperatures.
处理方案:	Treatments began 7 days after sowing. Plants were left intact, or leaf removal was conducted by peeling the entire coleoptile and first leaf from the culm to expose the axillary bud. The dissection was easily accomplished with minimal handling and without touching the bud. Axillary buds from the first leaf axil were harvested at 1, 3 and 6 h after leaf removal. Four replicates of 15 buds were harvested. The three highest quality replicates of each sample (by Bioanalyzer) were used for transcriptome analysis.
提取方案:	RNA was extracted using Trizol, the extracted RNA was digested with DNAseI and purified using an RNAeasy kit.
建库方案:	Libraries were constructed with the Illumina TruSeq kit and 125 bp reads were obtained from an Illumina HiSeq 2500 sequencer.

分子类型:	poly(A)+ RNA
库的片段类型:	SINGLE
库的链类型:	Forward
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2500
链特异性:	Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度