生长方案:	-
处理方案:	-
提取方案:	Four individual samples of dissociated cryopreserved cells (non-fiber cells only) obtained from a single human muscle biopsy were thawed based on the 10X Genomics Demonstrated Protocol (Manual Part Number CG00039 Rev C). Briefly, cryovials were removed from liquid nitrogen storage and immediately thawed in a 37℃ waterbath for 2 minutes. 1ml of the thawed cells were transferred into individual tubes, the cryovials were rinsed with 1ml of warm complete growth medium and the rinse medium was added dropwise (1 drop per 5 sec) to each tube containing the thawed cells. The cells were serially diluted with complete growth medium a total of 3 times by 1:1 volume dropwise additions with 1 minute wait before each addition. Next the cells were centrifuged at 300 rcf for 5 minutes and resuspended in complete growth medium. Cells were filtered through a 40μm cell strainer, counted, and the volume of medium was adjusted for the final concentration to be 1,000 cells/μl
建库方案:	Single-cell encapsulation with beads using the 10X Genomics Chromium Single Cell 3' v2 kit (PN 120235 and PN 120234 Module 1) was performed following manufacturer instructions (User Guide Rev A, 10X Genomics, Pleasanton, CA, USA). Briefly, 4 wells of a 10X microfluidic chip (PN 120236) were loaded each with an individual sample to target 5,000 cells per sample. Single-cell gel beads in emulsion (GEMs) were generated and reverse-transcription was performed in the emulsion prior to 12 cycles of cDNA amplification. Quality control and quantification of the amplified cDNA were assessed by Bioanalyzer. Libraries were constructed according to manufacturer instructions (PN 120234 Module 2). Each library was tagged with a different index for multiplexing (PN 120262) during sequencing. Library quality controls were assessed by Bioanalyzer and quantified by Qubit and quantitative PCR (KAPA Biosystems Quantification for Illumina platforms). Sequencing was performed on an HiSeq 2500 (Illumina, Inc., San Diego, CA) using the following read length: 26 bp Read1 (cell barcode(first 16 nt) + UMI(next 10 nt)), 8 bp i7 index (sample index), and 98 bp Read2 (insert).

分子类型:	poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	Forward
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2500
链特异性:	Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度