生长方案:	-
处理方案:	-
提取方案:	For each experiment, 4-mm punch biopsies were obtained from healthy whole skin specimens, immediately after resection from the inguinoiliac region of five male subjects. Samples were kept in MACS Tissue Storage Solution (Miltenyi Biotec) for no longer than 1 h before their enzymatical and mechanical dissociation with the Whole Skin Dissociation kit for human material (Miltenyi Biotec) and the Gentle MACS dissociator (Miltenyi Biotec), following the manufacturer's instructions. Cell suspensions were then filtered through 70-μm cell strainers (Falcon) and depleted of apoptotic and dead cells with the Dead Cell Removal Kit (Miltenyi Biotec).
建库方案:	Sequencing libraries were subsequently prepared following the Drop-seq methodology (Macosko et al., 2015), using a Chromium Single Cell Controller and the v2 chemistry from 10X Genomics. Thus, approximately 20,000 cells per sample were mixed with the retrotranscription reagents and pipetted into a Chip A Single Cell, also containing the Single Cell 3‘ Gel Bead suspension and Partitioning Oil. The Chip was subsequently loaded into a Chromium Single Cell Controller (10X Genomics) where the cells were captured in nanoscale droplets containing both the reagents needed for reverse transcription and a gel bead. Resulting Gel Bead-In-EMulsions (GEMs) were then transferred to a thermocycler in order to perform the retrotranscription following the manufacturer’s protocol. Each gel bead contained a specific 10X Genomics barcode, an Illumina R1 sequence, a Unique Molecular Identifier (UMI) and a poly-dT primer sequence. Therefore, from poly-adenylated mRNA the reaction produced full-length cDNA with a unique barcode per cell and transcript, which allowed tracing back all cDNA coming from each individual cell. Following an amplification step, cDNA was further processed by fragmentation, end repair and A-tailing double-sided size selection using AMPure XP beads. Finally, Illumina adaptors and a sample index were added through PCR using a total number of cycles adjusted to the cDNA concentration. After sample indexing, libraries were again subjected to double-sided size selection. Quantification of the libraries was carried out using the Qubit dsDNA HS Assay Kit (Life Technologies), and cDNA integrity was assessed using D1000 ScreenTapes (Agilent Technologies). Paired-end (26+74bp) sequencing (100 cycles) was finally performed with a HiSeq 4000 device (Illumina).

分子类型:	poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	Forward
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq X Ten
链特异性:	Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度