生长方案:	Before development, cells were grown in HL5 (Formedium, UK).
处理方案:	Cells were developed for 14 h on Whatman #50 filter paper in a humified chamber. Developed cells were disaggregated in KK2 + 20 mM EDTA then dedifferentiated in HL5 growth medium as a shaken suspension. Start times of development and dedifferentiation were staggered to allow simultaneous collection of samples into ice-cold KK2 at 0, 1, 2, 3, 4, 5 and 6 h of dedifferentiation.
提取方案:	Cell suspensions from different timepoints were pooled before loading onto the 10X Chromium Single Cell A Chip using the Chromium 3' Library and Gel Bead Kit v2. Samples were split between two chip inlets and treated as technical replicates
建库方案:	The purified GEM-RT product was subjected to 14 cycles of cDNA amplification. 35 microlitres of cDNA was used to prepare the 10X 3' RNA library, with 12/11 cycles used for sample index PCR. Final libraries were run on a NextSeq500 mid-output 150-cycle kit with a 26[8]98 cycle configuration to generate 130 million read pairs in total.

分子类型:	poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	NextSeq 500
链特异性:	-

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度