| Accession |
HRX536345 |
| title |
strt scRNA of gastric corpus from pancreatic ductal adenocarcinoma patient |
| BioProject |
PRJCA011342 |
| Study accession |
HRA002917 |
| Individual |
HRI273699 |
| Sample |
HRS437620 |
| Platform |
Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection
| Layout |
|
Briefly, first-strand cDNA was generated by a reverse-transcription reaction, followed by 18 cycles of PCR to amplify the cDNA. The amplified cDNA was pooled together before biotin tags were added to the 3’ end of the amplified cDNAs. We sheared the 300 ng of amplified cDNA into 300-bp fragments using a Covaris S2 system and used C1 beads to capture the 3’ terminal of the cDNA. The library was prepared using a KAPA Hyper Prep Kit (KAPA Biosystems). |
RNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| HRR742259 |
P51_NC_S1_L4_R1.fastq.gz
P51_NC_S1_L4_R2.fastq.gz
|
10,924.14
13,085.54
|
| HRR742258 |
P51_NC_S1_L3_R1.fastq.gz
P51_NC_S1_L3_R2.fastq.gz
|
9,675.28
11,348.42
|
| HRR742257 |
P51_NC_S1_L2_R1.fastq.gz
P51_NC_S1_L2_R2.fastq.gz
|
10,574.47
12,719.01
|
| HRR742256 |
P51_NC_S1_L1_R1.fastq.gz
P51_NC_S1_L1_R2.fastq.gz
|
15,427.35
17,849.72
|
|
|
Release date
|
2022-11-03
|
| Submitter |
Fuchou Tang (tangfuchou@pku.edu.cn)
|
| Organization |
Peking University |
| Submission date |
2022-08-29 |
