Total RNA was extracted using the RNeasy Mini Kit (QIAGEN) following the manufacturer’s instructions. We performed mRNA isolation using the NEB Poly (A) mRNA Magnetic Isolation Module (NEB) and constructed an RNA library using the KAPA Hyper Prep Kit (KAPA Biosystems). The RNA library was sequenced on an Illumina HiSeq 4000 platform using 150-bp paired-end sequencing.
RNA-Seq
TRANSCRIPTOMIC
PolyA
PAIRED
Processing
Planned read length (bp) for mate 1: 150 Planned read length (bp) for mate 2: 150
