| Accession |
HRX536362 |
| title |
siNME1_2_Gastric_2D_culture |
| BioProject |
PRJCA011342 |
| Study accession |
HRA002917 |
| Individual |
HRI273707 |
| Sample |
HRS437624 |
| Platform |
Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection
| Layout |
|
Total RNA was extracted using the RNeasy Mini Kit (QIAGEN) following the manufacturer’s instructions. We performed mRNA isolation using the NEB Poly (A) mRNA Magnetic Isolation Module (NEB) and constructed an RNA library using the KAPA Hyper Prep Kit (KAPA Biosystems). The RNA library was sequenced on an Illumina HiSeq 4000 platform using 150-bp paired-end sequencing. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| HRR742281 |
siRNA-1-siNME1-2_FKDL210173470-1a-AK6521-AK6673_1.fq.gz
siRNA-1-siNME1-2_FKDL210173470-1a-AK6521-AK6673_2.fq.gz
|
1,553.75
1,616.41
|
|
|
Release date
|
2022-11-03
|
| Submitter |
Fuchou Tang (tangfuchou@pku.edu.cn)
|
| Organization |
Peking University |
| Submission date |
2022-08-29 |
