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Experiment information
Accession CRX322644
Organism Macaca fascicularis
Title L-YF2-IP
BioProject PRJCA008042
BioSample SAMC559757
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
mRNA of cells form tissues was isolated by Oligo-dT beads and fragmented into 100 to 200 nt. After that, 1 ug fragmented RNA was subjected to the MeRIP procedure to enrich methylated RNA using anti m6A antibody. After immunoprecipitation, the enriched RNA was converted to cDNA by reverse transcription.Then Illumina adapters were ligated to DNA, and amplified by PCR. RIP-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
Release date2023-02-12
Run
Run accession Run data file information
File nameFile size (MB)
CRR375489 CRR375489.bam 4,775.75
SubmitterWeimin ci (ciwm@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2022-01-24