mRNA of cells form tissues was isolated by Oligo-dT beads and fragmented into 100 to 200 nt. After that, 1 ug fragmented RNA was subjected to the MeRIP procedure to enrich methylated RNA using anti m6A antibody. After immunoprecipitation, the enriched RNA was converted to cDNA by reverse transcription.Then Illumina adapters were ligated to DNA, and amplified by PCR.
RIP-Seq
TRANSCRIPTOMIC
PolyA
PAIRED
Processing
Planned read length (bp) for mate 1: 150 Planned read length (bp) for mate 2: 150 Insert size (bp): 200