| Accession | CRX470394 |
| Organism | Lycium barbarum |
| Title | Hi-C |
| BioProject | PRJCA010231 |
| BioSample | SAMC810313 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Two Hi-C libraries were performed as described by Chang Liu (2017) except for a few changes. Briefly in three steps, fresh tender leaves were fixed with 1% (v/v) formaldehyde and then nuclei isolated, Cross linking DNA was digested with MboI, Sticky overhangs were filled in with biotin-14-dCTP (Invitrogen, 19518018), blunt ends were ligated and proximity fragment formed chimeric junctions. Purified DNA was sheared to 200bp-500bp by Covaris S2. Chimeric fragments of biotin-labeled were captured through dynabeads streptavidin C1, which were created DNA library using NEBNext Ultra II DNA enzyme and corresponding adapters. |
Hi-C |
GENOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-10-12 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR530530 |
CRR530530_f1.fq.gz
CRR530530_r2.fq.gz
|
40,623.93
42,287.8
|
| CRR530531 |
CRR530531_f1.fq.gz
CRR530531_r2.fq.gz
|
40,962.81
45,500.3
|
|
| Submitter | Haiyan Yue (yue_yhy@ibp.ac.cn) |
|---|
| Organization | Institute of Biophysics, Chinese Academy of Sciences |
|---|
| Date submitted | 2022-07-11 |
|---|
