logo
Experiment information
Accession CRX634205
Organism Catalpa fargesii
Title G2_3
BioProject PRJCA014785
BioSample SAMC1148266
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according to the manufacturer’s protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and checked using RNase free agarose gel electrophoresis. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads. Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina(NEB #7530,New England Biolabs, Ipswich, MA, USA).The purified double-stranded cDNA fragments were end repaired, A base added, and ligated to Illumina sequencing adapters.The ligation reaction was purified with the AMPure XP Beads(1.0X).Ligated fragments were subjected to size selection by agarose gel electrophoresis and polymerase chain reaction (PCR) amplified.The resulting cDNA library was sequenced using Illumina Novaseq6010 by Gene Denovo Biotechnology Co. (Guangzhou, China). RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2023-06-03
Run
Run accession Run data file information
File nameFile size (MB)
CRR707878 CRR707878_f1.fastq.gz
CRR707878_r2.fastq.gz
1,729.3
1,820.85
SubmitterYu Zhang (zhangyu7009@126.com)
OrganizationChinese Academy of Forestry
Date submitted2023-03-08