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Experiment information
Accession CRX634218
Organism Catalpa fargesii
Title Y2_3IP
BioProject PRJCA014785
BioSample SAMC1148279
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according to the manufacturer’s protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and checked using RNase free agarose gel electrophoresis. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads. Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina(NEB #7530,New England Biolabs, Ipswich, MA, USA).The purified double-stranded cDNA fragments were end repaired, A base added, and ligated to Illumina sequencing adapters.The ligation reaction was purified with the AMPure XP Beads(1.0X).Ligated fragments were subjected to size selection by agarose gel electrophoresis and polymerase chain reaction (PCR) amplified.The resulting cDNA library was sequenced using Illumina Novaseq6023 by Gene Denovo Biotechnology Co. (Guangzhou, China). RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2023-06-03
Run
Run accession Run data file information
File nameFile size (MB)
CRR707891 CRR707891_f1.fastq.gz
CRR707891_r2.fastq.gz
1,940.21
2,121.39
SubmitterYu Zhang (zhangyu7009@126.com)
OrganizationChinese Academy of Forestry
Date submitted2023-03-08