Experiment information
Accession CRX859055
Organism biofilm metagenome
Title D2_cKO6
BioProject PRJCA021383
BioSample SAMC3176632
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
DNA of sorted NCSCs was extracted from the cell line using a QIAamp DNA Mini Kit, sheared to approximately 300-500 bp using a Covaris S220 instrument. Then the libraries were constructed through end-repair, A-tailing, adapter ligation and bisulfate-converted using a ZymoEZ DNA Methylation Kit AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2023-11-20
Run
Run accession Run data file information
File nameFile size (MB)
CRR947802 CRR947802_f1.fq.gz
CRR947802_r2.fq.gz
2,889.73
2,925.38
SubmitterLecheng Wei (lecwei@zju.edu.cn)
OrganizationZhejiang University
Date submitted2023-11-17
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Experiments(5)