| Accession | CRX1024258 |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Title | 8 |
| BioProject | PRJCA025203 |
| BioSample | SAMC3528473 |
| Platform | NextSeq CN500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA was extracted from all samples using a QIAamp® UCP Pathogen DNA Kit (Qiagen) following the manufacturer’s instructions. Human DNA was removed using Benzonase (Qiagen) and Tween20 (Sigma). Total RNA was extracted with a QIAamp® Viral RNA Kit (Qiagen) and ribosomal RNA was removed by a Ribo-Zero rRNA Removal Kit (Illumina). cDNA was generated using reverse transcriptase and dNTPs (Thermo Fisher). Libraries were constructed for the DNA and cDNA samples using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA)2. Library was quality assessed by Qubit dsDNA HS Assay kit followed by High Sensitivity DNA kit (Agilent) on an Agilent 2100 Bioanalyzer. Library pools were then loaded onto an Illumina Nextseq CN500 sequencer for 75 cycles of single-end sequencing to generate approximately 20 million reads for each library. For negative controls, we also prepared PBMC samples with 105 cells/mL from healthy donors in parallel with each batch, using the same protocol, and sterile deionized water was extracted alongside the specimens to serve as non-template controls (NTC). |
OTHER |
METAGENOMIC |
RANDOM |
SINGLE
|
|
| Processing |
Planned read length (bp): 50
|
| Release date | 2024-04-18 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1114932 |
CRR1114932.fastq.gz
|
52.7
|
|
| Submitter | Yuan Yang (yangyuan361@126.com) |
|---|
| Organization | Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital |
|---|
| Date submitted | 2024-04-17 |
|---|
