cDNA was fragmented and amplified, then the products were filtered twice. To construct the sequencing library, cDNA was fragmented, end-repaired and A-tailed. Then the adapter was ligated so the dual index PCR could amplify and distinguish these samples by different index sequences. The distribution of the main peak was between 200 and 600 bp and the libraries were sequenced in the same sequencing run.
OTHER
OTHER
unspecified
PAIRED
Processing
Planned read length (bp) for mate 1: 150 Planned read length (bp) for mate 2: 150