| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The Magnetic Soil and Stool DNA Kit was utilized for DNA extraction following the manufacturer's instructions. The complete 16S rRNA gene was amplified using the general primers 27F (5'-AGRGTTTGATYNTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3'), with the addition of PacBio barcode sequences unique to each sample. Library construction, sequencing, and data analysis were carried out using the PacBio Sequel II platform. |
AMPLICON |
METAGENOMIC |
PCR |
SINGLE
|
|
