| Accession | CRX1575079 |
| Organism | Mus musculus |
| Title | cKO placenta tissues for H3K27ac CUT&Tag in E12.5 |
| BioProject | PRJCA037685 |
| BioSample | SAMC4887164 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Fresh mouse placenta tissues were used for extracting nuclei with a nuclear extraction kit . Approximately 10,000 cell nuclei were prepared for CUT&Tag and library construction as previously described. The reagents used in experiments all came from the Hyperactive In-Situ ChIP Library Prep Kit for Illumina and the TurePrep Index Kit V2 for Illumina. All experimental procedures were performed according to the Vazyme user manual. Afterward, the sequence and bioinformatic analyses were conducted by the Novogene Bioinformatic Institute. |
OTHER |
GENOMIC |
other |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2025-03-25 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1702174 |
CRR1702174_r1.fastq.gz
CRR1702174_r2.fastq.gz
|
1,360.61
1,196.72
|
|
| Submitter | Zhongzhou Yang (zhongzhouyang@nju.edu.cn) |
|---|
| Organization | Nanjing University |
|---|
| Date submitted | 2025-03-24 |
|---|
