Basic information
Locus name	LOC_Os01g50770
Alias	SPL28
Organism	Rice (Oryza sativa)
Taxonomic identifier	[NCBI]
Function category	Protein degradation/modification
Effect for Senescence	delay
Gene Description	SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit μ1 (AP1M1), which is involved in the post-Golgi trafficking pathway. A green fluorescent protein (GFP) fusion protein of SPL28 (SPL28::GFP) localized to the Golgi apparatus.
Evidence	Molecular evidence:GFP [Ref 1]
References	1: Qiao Y, Jiang W, Lee J, Park B, Choi MS, Piao R, Woo MO, Roh JH, Han L, Paek NC, Seo HS, Koh HJ SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit micro 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). New Phytol. 2010 Jan;185(1):258-74
Gene Ontology	biological process intracellular protein transport vesicle-mediated transport cellular component clathrin adaptor complex
Sequence	LOC_Os01g50770.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutant name	spl28
Mutant/Transgenic	mutant
Ecotype	Hwacheongbyeo (an elite Korean japonica cultivar)
Mutagenesis type	N-methyl-N-nitrosourea (MNU) treatment
miRNA Interaction
Details	target: LOC_Os01g50770  miRNA: osa-miR1438 miRNA: osa-miR1438 mfe: -21.1 kcal/mol  p-value: 0.024053  position:  993  target 5' U               C A      U 3'             UUGAGAAUGAUAGAA U UAUCCU              AAUUUUUACUAUUUU A AUGGGA   miRNA  3'                            5'
Ortholog Group
Ortholog Groups: OG5_127474	Accession Taxon NP_172543 Arabidopsis thaliana NP_176277 Arabidopsis thaliana CMJ102C Cyanidioschyzon merolae strain 10D 182329 Chlamydomonas reinhardtii ACO61759 Micromonas sp. RCC299 NP_001044001 ( LOC_Os01g50770 ) Oryza sativa Japonica Group NP_001056201 Oryza sativa Japonica Group e_gw1.07.00.209.1 Ostreococcus tauri estExt_fgenesh1_pg.C_3280033 Physcomitrella patens subsp. patens estExt_fgenesh1_pm.C_3000002 Physcomitrella patens subsp. patens 27504.m000626 Ricinus communis 30147.m013894 Ricinus communis e_gw1.14.93.1 Thalassiosira pseudonana CCMP1335 XP_002945806 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR11998 2.5E-270 4 429 No hit NA PIRSF PIRSF005992 5.5E-216 4 428 IPR001392 Clathrin adaptor, mu subunit Pfam PF01217 1.2E-5 5 132 IPR022775 AP complex, mu/sigma subunit SUPERFAMILY SSF64356 8.2E-36 5 142 IPR011012 Longin-like domain PRINTS PR00314 1.6E-64 15 35 IPR001392 Clathrin adaptor, mu subunit PRINTS PR00314 1.6E-64 104 131 IPR001392 Clathrin adaptor, mu subunit Pfam PF00928 8.2E-92 160 427 IPR008968 Clathrin adaptor, mu subunit, C-terminal ProSitePatterns PS00990 NA 160 180 IPR018240 Clathrin adaptor, mu subunit, conserved site SUPERFAMILY SSF49447 6.5E-96 160 427 IPR008968 Clathrin adaptor, mu subunit, C-terminal PRINTS PR00314 1.6E-64 162 190 IPR001392 Clathrin adaptor, mu subunit ProSiteProfiles PS51072 100.2 168 428 IPR008968 Clathrin adaptor, mu subunit, C-terminal PRINTS PR00314 1.6E-64 237 264 IPR001392 Clathrin adaptor, mu subunit ProSitePatterns PS00991 NA 257 271 IPR018240 Clathrin adaptor, mu subunit, conserved site PRINTS PR00314 1.6E-64 306 321 IPR001392 Clathrin adaptor, mu subunit PRINTS PR00314 1.6E-64 346 357 IPR001392 Clathrin adaptor, mu subunit