The pituitary gonadotropin content in Clarias batrachus was determined by radiophosphorus assay in chicks. The levels in spawning male, post spawning male, spawning female and post spawning female were found to be 65.66, 27.7, 36.69 and 49.98 micrograms LH activity/kg body weight respectively. The gonadosomatic index (GSI) in the above groups was found to be 0.3599, 0.0763, 9.2327 and 0.4334 respectively. Correlation between the gonadotropins and GSI was also worked out but was found to be statistically non-significant (P > 0.05).