Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages.

B Micheel, S Heymann, G Scharte, V Böttger, F Vogel, S Dübel, F Breitling, M Little, O Behrsing
Author Information
  1. B Micheel: Antibody Modification Group, Max Delbrück Centre for Molecular Medicine, Berlin-Buch, Germany.

Abstract

Three monoclonal antibodies (MAbs) were produced which react with epitopes of the main structural coat protein (pVIII) of filamentous fd phages as demonstrated by solid-phase fluorometric enzyme immunoassays and by immunoelectron microscopy. The antibodies are of the IgG1, IgG2a and IgG2b immunoglobulin subclasses. Since they also react with recombinant phages expressing antigen fragments in their pIII region they may be suitable reagents for the demonstration and isolation of filamentous phages used in recombinant protein technology.

MeSH Term

Animals
Antibodies
Antibodies, Monoclonal
Binding Sites, Antibody
Capsid
Clone Cells
Epitopes
Hybrid Cells
Inovirus
Mice
Mice, Inbred BALB C
Microscopy, Immunoelectron

Chemicals

Antibodies
Antibodies, Monoclonal
Epitopes

Word Cloud

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