Smoking, gender, and survival association with allele loss for the LOH11B lung cancer region on chromosome 11.

G Schreiber, K M Fong, B Peterson, B E Johnson, K C O'Briant, G Bepler
Author Information
  1. G Schreiber: Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.

Abstract

We have reported frequent allele loss for the marker HRAS on chromosome 11p in human lung cancer and defined the smallest common region of deletion (designated LOH11B) to approximately 500 kb. Here, we investigated the association of allele loss for LOH11B with epidemiological, pathological, and clinical parameters. Analysis of allele loss was performed using Southern blotting on a cohort of 200 patients with lung cancer, and data were interpreted with the use of a phosphorimager. Results were statistically compared with retrospectively collected variables. LOH11B allele loss was significantly associated with cigarette consumption (P = 0.009), gender (P = 0.02), and survival (P = 0.04). None of the nonsmokers had allele loss as compared with 28% of the patients with low and 43% with high cigarette consumption. Allele loss was more frequent in men (43%) than in women (11%). The median survival of patients without allele loss was 42 months compared with 25 months for patients with allele loss. These results suggest that the LOH11B region contains a gene responsible for a more malignant phenotype independent of the metastatic potential of lung cancer. They also suggest that alterations in this gene are associated with cigarette consumption and are more frequent in men than in women.

MeSH Term

Adult
Aged
Aged, 80 and over
Alleles
Blotting, Southern
Cause of Death
Cell Transformation, Neoplastic
Chromosome Deletion
Chromosome Mapping
Chromosomes, Human, Pair 11
Female
Genetic Markers
Humans
Lung Neoplasms
Male
Middle Aged
Phenotype
Sex Factors
Smoking
Survival Analysis

Chemicals

Genetic Markers

Word Cloud

Created with Highcharts 10.0.0lossalleleLOH11BlungcancerpatientsfrequentregioncomparedcigaretteconsumptionP=0survivalchromosomeassociationassociatedgender43%menwomenmonthssuggestgenereportedmarkerHRAS11phumandefinedsmallestcommondeletiondesignatedapproximately500kbinvestigatedepidemiologicalpathologicalclinicalparametersAnalysisperformedusingSouthernblottingcohort200datainterpretedusephosphorimagerResultsstatisticallyretrospectivelycollectedvariablessignificantly0090204Nonenonsmokers28%lowhighAllele11%medianwithout4225resultscontainsresponsiblemalignantphenotypeindependentmetastaticpotentialalsoalterationsSmoking11

Similar Articles

Cited By (2)