Effect of hyperhomocystinemia and hypertension on endothelial function in methylenetetrahydrofolate reductase-deficient mice.

Agostino Virdis, Marc Iglarz, Mario Fritsch Neves, Farhad Amiri, Rhian M Touyz, Rima Rozen, Ernesto L Schiffrin
Author Information
  1. Agostino Virdis: Multidisciplinary Research Group on Hypertension, Clinical Research Institute of Montreal, University of Montreal, Montreal, Quebec, Canada.

Abstract

OBJECTIVE: We evaluated the effect of hyperhomocystinemia and angiotensin (Ang) II on vascular function and structure in methylenetetrahydrofolate reductase knockout mice (Mthfr+/-).
METHODS AND RESULTS: Mthfr+/- and controls (Mthfr+/+) received Ang II (400 ng/kg per min SC) or saline (14 days). Blood pressure, similar in Mthfr+/- and Mthfr+/+, was increased by Ang II. Acetylcholine- and bradykinin-induced relaxations were impaired in mesenteric resistance arteries (pressurized myograph) in Mthfr+/- and in Ang II-infused Mthfr+/+ mice and additionally blunted in Ang II-infused Mthfr+/- mice. The inhibition by L-NAME on Acetylcholine was reduced in Mthfr+/- and in Ang II-Mthfr+/+ and absent in Ang II-Mthfr+/- mice. In these groups, vitamin C improved the response to Acetylcholine and restored the inhibition by L-NAME. The media to lumen ratio of small arteries, similar in Mthfr+/- and Mthfr+/+, was increased by Ang II. Vascular NADPH oxidase activity, similar in Mthfr+/- and Mthfr+/+, increased after Ang II infusion. Vascular xanthine oxidase activity was also similar in Mthfr+/- and Mthfr+/+. Superoxide production in the aorta was reduced by sepiapterin and by L-NAME, suggesting that reduced bioavailability of tetrahydrobiopterin and uncoupling of nitric oxide synthase were the origin of increased reactive oxygen species in this model.
CONCLUSIONS: Mthfr+/- mice show endothelial dysfunction of mesenteric vessels probably attributable to a reduced nitric oxide bioavailability caused by oxidative excess due to uncoupling of nitric oxide synthase without vascular structural alterations. Concurrent Ang II-induced hypertension additionally reduced nitric oxide, increased NADPH oxidase activity, and induced structural alterations. Our findings suggest additive adverse effect of Ang II-dependent hypertension and hyperhomocystinemia on endothelial function.

MeSH Term

Acetylcholine
Angiotensin II
Animals
Aorta
Biological Availability
Endothelium, Vascular
Hyperhomocysteinemia
Hypertension
In Vitro Techniques
Mesenteric Arteries
Methylenetetrahydrofolate Reductase (NADPH2)
Mice
Mice, Knockout
NADPH Oxidases
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Superoxides
Vasodilation
Xanthine Oxidase

Chemicals

Superoxides
Angiotensin II
Nitric Oxide
Nitric Oxide Synthase
Xanthine Oxidase
Methylenetetrahydrofolate Reductase (NADPH2)
NADPH Oxidases
Acetylcholine
NG-Nitroarginine Methyl Ester

Word Cloud

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