Evaluation of the EVIGENE VRE detection kit for detection of vanA and vanB genes in vancomycin-resistant enterococci.
Abdullah Kilic, Mehmet Baysallar, Gul Bahar, Ayten Kucukkaraaslan, Feriha Cilli, Levent Doganci
Author Information
Abdullah Kilic: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Mehmet Baysallar: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Gul Bahar: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Ayten Kucukkaraaslan: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Feriha Cilli: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Levent Doganci: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
The aim of this study was to evaluate the performance of the EVIGENE VRE Detection kit and compare it with PCR, considered the gold standard for detection of vancomycin-resistant enterococci (VRE). The correlation between the MIC values of vancomycin and teicoplanin using the epsilon test was also determined. In the EVIGENE VRE Detection kit, DNA probes specific for bacterial target DNA sequences are bound to microwell plates. A hundred and ten VRE (104 Enterococcus faecium and six Enterococcus faecalis) and 45 vancomycin-susceptible E. faecium were tested. All VRE strains were found to be positive for the vanA genotype using the EVIGENE VRE Detection kit. All results obtained with the EVIGENE VRE Detection kit were confirmed by PCR. MIC results for the strains also correlated highly with the PCR and kit results. The EVIGENE VRE Detection kit should be used in preference to other methods for detecting resistance genes in all strains, since it is less time-consuming, does not require the handling of hazardous chemicals and has the same specificity as PCR.
