- Hua-Li Cui: School of Life Science, Anhui University, Hefei 230039, China. huali8782@126.com
OBJECTIVE: To optimize the conditions for exopolysaccharides (EPS) production by Morchella esculenta GIM 5.69.
METHODS: A Plackett-Burman design was used to evaluate the effects of variables factors. The optimal combined conditions for maximum polysaccharide production were further optimized by response surface methodology.
RESULTS: The optimal fermentation conditions were determined as follows: glucose 30%, cultivation time 5.98 d, rotary speed 217.44 r/min. The average yield of polysaccharide validation experiments was 53.04%, the relative error was 1.64%.
CONCLUSION: This research has the vital significance regarding the Morchella esculenta polysaccharide function of the product development and the application.