A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR.
Kristen N Hellein, Elizabeth M Kennedy, Valerie J Harwood, Katrina V Gordon, Shiao Y Wang, Joe Eugene Lepo
Author Information
Kristen N Hellein: Center for Environmental Diagnostics and Bioremediation, University of West Florida, 11000 University Parkway, Pensacola, FL 32514, USA.
Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings.
