Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae.

Sandrine Bernabeu, Laurent Poirel, Patrice Nordmann
Author Information
  1. Sandrine Bernabeu: Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France

Abstract

Carbapenem-hydrolyzing ß-lactamases are the most powerful ß-lactamases being able to hydrolyse almost all ß-lactams. They are mostly of the KPC, VIM, IMP, NDM, and OXA-48 type. A spectrophotometry technique based on analysis of the imipenem hydrolysis has been developed that differentiated carbapenemase- from noncarbapenemase producers. This inexpensive technique adapted to screening of carbapenemase producers may be implemented in any reference laboratory worldwide.

MeSH Term

Bacterial Proteins
Bacteriological Techniques
Enterobacteriaceae
Humans
Hydrolysis
Imipenem
Spectrophotometry
beta-Lactamases

Chemicals

Bacterial Proteins
Imipenem
beta-Lactamases
carbapenemase

Word Cloud

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