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Genetics
08, 2017;206 (4): 2207-2223.

An Essential Regulatory System Originating from Polygenic Transcriptional Rewiring of PhoP-PhoQ of .

Bao-Yu Peng, Yue Pan, Ru-Jiao Li, Jin-Wei Wei, Fang Liang, Li Wang, Fang-Fang Wang, Wei Qian
Author Information
  1. Bao-Yu Peng: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
  2. Yue Pan: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
  3. Ru-Jiao Li: Beijing Institute of Genomics, Chinese Academy of Sciences, 100101, China.
  4. Jin-Wei Wei: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
  5. Fang Liang: Beijing Institute of Genomics, Chinese Academy of Sciences, 100101, China.
  6. Li Wang: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
  7. Fang-Fang Wang: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
  8. Wei Qian: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China qianw@im.ac.cn. ORCID
PMID: 28550013 DOI: 10.1534/genetics.117.200204

Abstract

How essential, regulatory genes originate and evolve is intriguing because mutations of these genes not only lead to lethality in organisms, but also have pleiotropic effects since they control the expression of multiple downstream genes. Therefore, the evolution of essential, regulatory genes is not only determined by genetic variations of their own sequences, but also by the biological function of downstream genes and molecular mechanisms of regulation. To understand the origin of essential, regulatory genes, experimental dissection of the complete regulatory cascade is needed. Here, we provide genetic evidences to reveal that PhoP-PhoQ is an essential two-component signal transduction system in the gram-negative bacterium , but that its orthologs in other bacteria belonging to Proteobacteria are nonessential. Mutational, biochemical, and chromatin immunoprecipitation together with high-throughput sequencing analyses revealed that and of and its close relative are replaceable, and that the consensus binding motifs of the transcription factor PhoP are also highly conserved. PhoP in regulates the transcription of a number of essential, structural genes by directly binding to -regulatory elements (CREs); however, these CREs are lacking in the orthologous essential, structural genes in , and thus the regulatory relationships between PhoP and these downstream essential genes are disassociated. Our findings suggested that the recruitment of regulatory proteins by critical structural genes via transcription factor-CRE rewiring is a driving force in the origin and functional divergence of essential, regulatory genes.

Keywords

PhoP-PhoQ Xanthomonas campestris pv. campestris essential gene transcription-cis regulatory element rewiring two-component signal transduction system

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MeSH Term

Bacterial Proteins
Gene Expression Regulation, Bacterial
Regulatory Sequences, Nucleic Acid
Transcriptional Activation
Xanthomonas campestris

Chemicals

Bacterial Proteins
PhoQ protein, Bacteria
PhoP protein, Bacteria
Journal Article
Article has an altmetric score of 1

Word Cloud

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