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08 18, 2017;7 (1): 8792.

Molecular bases for differential aging programs between flag and second leaves during grain-filling in rice.

Shinyoung Lee, Hyobin Jeong, Sichul Lee, Jinwon Lee, Sun-Ji Kim, Ji-Won Park, Hye Ryun Woo, Pyung Ok Lim, Gynheung An, Hong Gil Nam, Daehee Hwang
Author Information
  1. Shinyoung Lee: Center for Plant Ageing Research, IBS, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea.
  2. Hyobin Jeong: Center for Plant Ageing Research, IBS, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea.
  3. Sichul Lee: Center for Plant Ageing Research, IBS, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea.
  4. Jinwon Lee: Center for Plant Ageing Research, IBS, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea.
  5. Sun-Ji Kim: Center for Plant Ageing Research, IBS, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea.
  6. Ji-Won Park: Center for Plant Ageing Research, IBS, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea.
  7. Hye Ryun Woo: Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea.
  8. Pyung Ok Lim: Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea.
  9. Gynheung An: Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, 446-701, Republic of Korea.
  10. Hong Gil Nam: Center for Plant Ageing Research, IBS, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea. nam@dgist.ac.kr.
  11. Daehee Hwang: Center for Plant Ageing Research, IBS, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, Republic of Korea. dhwang@dgist.ac.kr.
PMID: 28821707 DOI: 10.1038/s41598-017-07035-9

Abstract

Flag leaves (FL) and second leaves (SL) in rice show differential aging patterns during monocarpic senescence. Coordination of aging programs between FL and SL is important for grain yield and quality. However, the molecular bases for differential aging programs between FL and SL have not been systematically explored in rice. Here, we performed mRNA-sequencing of FL and SL at six time points during grain-filling and identified four molecular bases for differential aging programs between FL and SL: phenylpropanoid biosynthesis, photosynthesis, amino acid (AA) transport, and hormone response. Of them, photosynthesis (carbon assimilation) and AA transport (nitrogen remobilization) predominantly occurred in FL and SL, respectively, during grain-filling. Unlike other molecular bases, AA transport showed consistent differential expression patterns between FL and SL in independent samples. Moreover, long-distance AA transporters showed invariant differential expression patterns between FL and SL after panicle removal, which was consistent to invariant differential nitrogen contents between FL and SL after panicle removal. Therefore, our results suggest that the supplies of carbon and nitrogen to seeds is functionally segregated between FL and SL and that long-distance AA transport is an invariant core program for high nitrogen remobilization in SL.

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MeSH Term

Chlorophyll
Edible Grain
Gene Expression Profiling
Gene Expression Regulation, Plant
Models, Biological
Nitrogen
Oryza
Photosynthesis
Plant Leaves
Plant Physiological Phenomena
RNA, Messenger
Transcriptome

Chemicals

RNA, Messenger
Chlorophyll
Nitrogen
Journal Article Research Support, Non-U.S. Gov't

Links to CNCB-NGDC Resources

GEN: GEND000260 (Fundamental molecular bases for differential transcriptional programs of top leaves during grain-filling in rice)

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