Aerobic exercise inhibits acute lung injury: from mouse to human evidence Exercise reduced lung injury markers in mouse and in cells.
Nicole Cristine Rigonato-Oliveira, BreAnne Mackenzie, Andre Luis Lacerda Bachi, Manoel Carneiro Oliveira-Junior, Alana Santos-Dias, Maysa Alves Rodrigues Brandao-Rangel, Humberto Delle, Tamara Costa-Guimaraes, Nilsa Regina Damaceno-Rodrigues, Nilsa Regina Dulley, Marcela Anhesini Benetti, Christiane Malfitano, Christiane de Angelis, Regiane Albertini, Ana Paula Ligeiro Oliveira, Asghar Abbasi, Hinnak Northoff, Rodolfo Paula Vieira
Author Information
Nicole Cristine Rigonato-Oliveira: Nove de Julho University, São Paulo - SP, Brazil.
BreAnne Mackenzie: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil.
Andre Luis Lacerda Bachi: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil.
Manoel Carneiro Oliveira-Junior: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil.
Alana Santos-Dias: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil.
Maysa Alves Rodrigues Brandao-Rangel: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil. Federal University of São Paulo (UNIFESP), Post-graduation Program in Sciences of Human Movement and Rehabilitation, Santos - SP, Brazil.
Humberto Delle: Nove de Julho University, São Paulo - SP, Brazil.
Tamara Costa-Guimaraes: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil. Universidade Brasil, São Paulo - SP, Brazil.
Nilsa Regina Damaceno-Rodrigues: University of Sao Paulo, School of Medicine, Department of Pathology (LIM 59), Sao Paulo - SP, Brazil.
Nilsa Regina Dulley: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil.
Marcela Anhesini Benetti: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil.
Christiane Malfitano: Science Department of Health, Universidade Federal de Lavras (UFLA), Lavras - MG, Brazil.
Christiane de Angelis: Federal University of São Paulo (UNIFESP), Campus São Paulo, São Paulo - SP, Brazil.
Regiane Albertini: Federal University of São Paulo (UNIFESP), Post-graduation Program in Sciences of Human Movement and Rehabilitation, Santos - SP, Brazil.
Ana Paula Ligeiro Oliveira: Nove de Julho University, São Paulo - SP, Brazil.
Asghar Abbasi: Division of Respiratory & Critical Care Physiology & Medicine, Los Angeles Biomedical Research Institute at Harbor - UCLA Medical Center, California - CA, USA.
Hinnak Northoff: Institute of Clinical and Experimental Transfusion Medicine (IKET), University of Tübingen, Tübingen, Germany.
Rodolfo Paula Vieira: Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos - SP, Brazil. Federal University of São Paulo (UNIFESP), Post-graduation Program in Sciences of Human Movement and Rehabilitation, Santos - SP, Brazil. Universidade Brasil, São Paulo - SP, Brazil.
Acute respiratory distress syndrome (ARDS) is defined as hypoxemic respiratory failure with intense pulmonary inflammation, involving hyperactivation of endothelial cells and neutrophils. Given the anti-inflammatory effects of aerobic exercise (AE), this study investigated whether AE performed daily for 5 weeks would inhibit extra-pulmonary LPS-induced ARDS. C57Bl/6 mice were distributed into Control, Exercise, LPS and Exercise+LPS groups. AE was performed on a treadmill for 5x/week for four weeks before LPS administration. 24hours after the final AE physical test, animals received 100ug of LPS intra-peritoneally. In addition, whole blood cell culture, neutrophils and human endothelial cells were preincubated with IL-10, an anti-inflammatory cytokine induced by exercise. AE reduced total protein levels (p<0.01) and neutrophil accumulation in bronchoalveolar lavage (BAL) (p<0.01) and lung parenchyma (p<0.01). AE reduced BAL inflammatory cytokines IL-1β, IL-6 and GM-CSF (p<0.001), CXCL1/KC, IL-17, TNF-alpha and IGF-1 (p<0.01). Systemically, AE reduced IL-1β, IL-6 and IFN-gamma (p<0.001), CXCL1/KC (p<0.01) and TNF-alpha (p<0.05). AE increased IL-10 levels in serum (p<0.001) and BAL (p<0.001). Furthermore, AE increased superoxide dismutase SOD (p<0.01) and decreased superoxide anion accumulation in the lungs (p<0.01). Lastly, pre-incubation with IL-10 significantly reduced LPS-induced activation of whole blood cells, neutrophils and HUVECs, as observed by reduced production of IL-1β, IL-6, IL-8 and TNF-alpha. Our data suggest that AE inhibited LPS-induced lung inflammation by attenuating inflammatory cytokines and oxidative stress markers in mice and human cell culture via enhanced IL-10 production.
