Single-cell RNA sequencing reveals chemokine self-feeding of myeloma cells promotes extramedullary metastasis.
Shuang Geng, Jing Wang, Xiannian Zhang, Jia-Jia Zhang, Fan Wu, Yuhong Pang, Yuping Zhong, Jianbin Wang, Wenming Wang, Xiaoqing Lyu, Yanyi Huang, Hongmei Jing
Author Information
- Shuang Geng: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China.
- Jing Wang: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China.
- Xiannian Zhang: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China.
- Jia-Jia Zhang: Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
- Fan Wu: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China.
- Yuhong Pang: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China.
- Yuping Zhong: Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
- Jianbin Wang: School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
- Wenming Wang: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China.
- Xiaoqing Lyu: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China.
- Yanyi Huang: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China.
- Hongmei Jing: Department of Hematology, Biodynamic Optical Imaging Center (BIOPIC) and Lymphoma Research Center, Third Hospital, Peking University, Beijing, China. ORCID
In this study, we aimed to determine the mechanisms underlying the initial extramedullary translocation of myeloma cells from bone marrow into peripheral blood. We found that clonal circulating plasma cells (cPCs) are more frequently detected by flow cytometry in extramedullary plasmacytoma (EMP) patients and worsen their prognosis. It is technically much easier to collect single cPCs using FACS than it is to perform EMP biopsy. Therefore, combining EMP imaging with cPC detection may be a promising strategy for prognostic stratification. Here, using single-cell transcriptome analysis, we found that the chemokine CXCL12, a key molecule involved in CXCR4-dependent cell retention in the bone marrow, is abnormally upregulated in cPCs and might initially enable cPCs to evade bone marrow retention and translocate into the bloodstream.
Aged
Bone Marrow
Chemokines
Female
Humans
Male
Middle Aged
Multiple Myeloma
Neoplasm Metastasis
Phenotype
Prognosis
Sequence Analysis, RNA
Single-Cell Analysis