The Transcriptional Landscape of Polyploid Wheats and Their Diploid Ancestors during Embryogenesis and Grain Development.
Daoquan Xiang, Teagen D Quilichini, Ziying Liu, Peng Gao, Youlian Pan, Qiang Li, Kirby T Nilsen, Prakash Venglat, Eddi Esteban, Asher Pasha, Yejun Wang, Rui Wen, Zhongjuan Zhang, Zhaodong Hao, Edwin Wang, Yangdou Wei, Richard Cuthbert, Leon V Kochian, Andrew Sharpe, Nicholas Provart, Dolf Weijers, C Stewart Gillmor, Curtis Pozniak, Raju Datla
Author Information
Daoquan Xiang: Aquatic and Crop Resource Development, National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada daoquan.xiang@nrc-cnrc.gc.ca raju.datla@nrc-cnrc.gc.ca. ORCID
Teagen D Quilichini: Aquatic and Crop Resource Development, National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada. ORCID
Ziying Liu: Digital Technologies Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada. ORCID
Peng Gao: Aquatic and Crop Resource Development, National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada. ORCID
Youlian Pan: Digital Technologies Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada. ORCID
Qiang Li: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China. ORCID
Kirby T Nilsen: Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A8, Canada. ORCID
Prakash Venglat: Department of Plant Sciences and Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A8, Canada. ORCID
Eddi Esteban: Department of Cell and Systems Biology/Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario M5S 3B2, Canada. ORCID
Asher Pasha: Department of Cell and Systems Biology/Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario M5S 3B2, Canada. ORCID
Yejun Wang: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Shenzhen University, Shenzhen 518060, China. ORCID
Rui Wen: Aquatic and Crop Resource Development, National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada. ORCID
Zhongjuan Zhang: Laboratory of Biochemistry, Wageningen University, 6703HA Wageningen, The Netherlands. ORCID
Zhaodong Hao: Laboratory of Biochemistry, Wageningen University, 6703HA Wageningen, The Netherlands. ORCID
Edwin Wang: Cumming School of Medicine, University of Calgary, Calgary, Alberta T2N 1N4, Canada. ORCID
Yangdou Wei: College of Art and Science, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A5, Canada. ORCID
Richard Cuthbert: Agriculture and Agri-Food Canada, Swift Current, Saskatchewan S9H 3X2, Canada. ORCID
Leon V Kochian: Global Institute for Food Security, University of Saskatchewan, Saskatoon, Saskatchewan S7N 4J8, Canada. ORCID
Andrew Sharpe: Global Institute for Food Security, University of Saskatchewan, Saskatoon, Saskatchewan S7N 4J8, Canada. ORCID
Nicholas Provart: Department of Cell and Systems Biology/Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario M5S 3B2, Canada. ORCID
Dolf Weijers: Laboratory of Biochemistry, Wageningen University, 6703HA Wageningen, The Netherlands. ORCID
C Stewart Gillmor: Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato 36824, México. ORCID
Curtis Pozniak: Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A8, Canada. ORCID
Raju Datla: Aquatic and Crop Resource Development, National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada daoquan.xiang@nrc-cnrc.gc.ca raju.datla@nrc-cnrc.gc.ca. ORCID
Modern wheat production comes from two polyploid species, and (var ), which putatively arose from diploid ancestors , , and How gene expression during embryogenesis and grain development in wheats has been shaped by the differing contributions of diploid genomes through hybridization, polyploidization, and breeding selection is not well understood. This study describes the global landscape of gene activities during wheat embryogenesis and grain development. Using comprehensive transcriptomic analyses of two wheat cultivars and three diploid grasses, we investigated gene expression at seven stages of embryo development, two endosperm stages, and one pericarp stage. We identified transcriptional signatures and developmental similarities and differences among the five species, revealing the evolutionary divergence of gene expression programs and the contributions of A, B, and D subgenomes to grain development in polyploid wheats. The characterization of embryonic transcriptional programming in hexaploid wheat, tetraploid wheat, and diploid grass species provides insight into the landscape of gene expression in modern wheat and its ancestral species. This study presents a framework for understanding the evolution of domesticated wheat and the selective pressures placed on grain production, with important implications for future performance and yield improvements.plantcell;31/12/2888/FX1F1fx1.
