Measurement of aflatoxin M1 in powder and pasteurized milk samples by using a label-free electrochemical aptasensor based on platinum nanoparticles loaded on Fe-based metal-organic frameworks.
Fahime Jahangiri-Dehaghani, Hamid R Zare, Zahra Shekari
Author Information
Fahime Jahangiri-Dehaghani: Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran.
Hamid R Zare: Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran. Electronic address: hrzare@yazd.ac.ir.
Zahra Shekari: Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran.
In the present study, a sensitive label-free electrochemical aptasensor is introduced to measure aflatoxin M1 (AFM1) by using platinum nanoparticles (PtNPs) decorated on a glassy carbon electrode (GCE) modified with Fe-based metal-organic frameworks, MIL-101(Fe). The MIL-101(Fe) and the PtNP/MIL-101(Fe) are synthesized and characterized by Fourier transform infrared spectroscopy, X-ray diffraction, UV-Visible spectroscopy, and field-emission scanning electron microscopy. Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are done to monitor the fabrication processes of the aptasensor. In optimum conditions, the linear calibration range of 1.0 × 10 to 80.0 ng mL and the detection limit of 2.0 × 10 ng mL are obtained to measure AFM1 concentration using the EIS method. Finally, the fabricated aptasensor is successfully applied to measure AFM1 concentration in powder and pasteurized milk samples.
