M-CSF- and L929-derived macrophages present distinct metabolic profiles with similar inflammatory outcomes.
Lauar de Brito Monteiro, Gustavo Gastão Davanzo, Cristhiane Fávero de Aguiar, Felipe Corrêa da Silva, Jessica Rodrigues de Andrade, Ana Campos Codo, Jessica Aparecida da Silva Pereira, Leonardo Pimentel de Freitas, Pedro Manoel Moraes-Vieira
Author Information
Lauar de Brito Monteiro: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil.
Gustavo Gastão Davanzo: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil.
Cristhiane Fávero de Aguiar: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil.
Felipe Corrêa da Silva: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil.
Jessica Rodrigues de Andrade: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil.
Ana Campos Codo: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil.
Jessica Aparecida da Silva Pereira: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil; Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, SP, Brazil.
Leonardo Pimentel de Freitas: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil.
Pedro Manoel Moraes-Vieira: Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil; Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, SP, Brazil; Division of Metabolism, Experimental Medicine Research Cluster (EMRC), University of Campinas, Campinas, SP, Brazil. Electronic address: pmvieira@unicamp.br.
Macrophages are essential components of the immune system. Macrophages can be derived from the bone marrow of mice with either recombinant M-CSF or L929 supernatant. Recent literature considers recombinant M-CSF- and L929-derived macrophages as equals, even though L929-derived macrophages are exposed to other substances secreted in the L929 supernatant, and not only M-CSF. Thus, we decided to perform a comparative analysis of both inflammatory and metabolic profiles of macrophages differentiated under the aforementioned conditions, which is relevant for standardization and interpretation of in vitro studies. We observed that, when treated with LPS, L929macs secrete lower levels of proinflammatory cytokines (TNF-α, IL-6, IL12) and present higher glycolysis and oxygen consumption when compared with M-CSFmacs. L929macs also have increased mitochondrial mass, with higher percentage of dysfunctional mitochondria. This sort of information can help direct further studies towards a more specific approach for macrophage generation.
