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05 29, 2020;40 (5):

Brevinin-1GHd: a novel Hylarana guentheri skin secretion-derived Brevinin-1 type peptide with antimicrobial and anticancer therapeutic potential.

Yangyang Jiang, Yue Wu, Tao Wang, Xiaoling Chen, Mei Zhou, Chengbang Ma, Xinping Xi, Ying Zhang, Tianbao Chen, Chris Shaw, Lei Wang
Author Information
  1. Yangyang Jiang: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  2. Yue Wu: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  3. Tao Wang: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  4. Xiaoling Chen: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  5. Mei Zhou: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  6. Chengbang Ma: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  7. Xinping Xi: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  8. Ying Zhang: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  9. Tianbao Chen: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  10. Chris Shaw: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
  11. Lei Wang: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, Northern Ireland, U.K.
PMID: 32347293 DOI: 10.1042/BSR20200019

Abstract

Host-defense antimicrobial peptides (AMPs) from amphibians are usually considered as one of the most promising next-generation antibiotics because of their excellent antimicrobial properties and low cytotoxicity. In the present study, one novel Brevinin-1 type peptide, Brevinin-1GHd, was isolated and characterized from the skin secretion of the frog, Hylarana guentheri. Brevinin-1GHd was found to possess a wide range of antimicrobial activity through penetrating the bacterial membrane within a short time while showing low hemolysis at bactericidal concentrations, even against the resistant strains. It also inhibited and eradicated biofilms that are thought to be closely related to the rise in resistance. Meanwhile, Brevinin-1GHd exhibited wide-spectrum anti-proliferation activity toward human cancer lines. Taken together, these results indicate that Brevinin-1GHd with its excellent antimicrobial and anticancer activities is a promising candidate for a novel antibiotic agent, and study of its structure-activity relationships also provided a rational template for further research and peptide analog design.

Keywords

Brevinin-1GHd anti-proliferation antimicrobial peptides (AMPs) biofilms resistant strains

References

  1. Biochim Biophys Acta. 2009 Aug;1788(8):1639-55 [PMID: 19272309]
  2. J Pept Sci. 2019 Nov;25(11):e3208 [PMID: 31721374]
  3. Biochem J. 2002 Nov 15;368(Pt 1):171-82 [PMID: 12164787]
  4. Biochemistry. 2003 Dec 2;42(47):14023-35 [PMID: 14636071]
  5. Eur Biophys J. 2011 Apr;40(4):555-64 [PMID: 21312033]
  6. Biochem Biophys Res Commun. 1992 Nov 30;189(1):184-90 [PMID: 1449472]
  7. J Pept Res. 2001 Nov;58(5):433-41 [PMID: 11892852]
  8. PLoS One. 2015 May 21;10(5):e0124210 [PMID: 25997127]
  9. J Biol Chem. 1994 Apr 8;269(14):10849-55 [PMID: 8144672]
  10. Molecules. 2017 Oct 24;22(10): [PMID: 29064402]
  11. Front Microbiol. 2018 Nov 28;9:2846 [PMID: 30555431]
  12. J Nat Prod. 2014 Nov 26;77(11):2410-7 [PMID: 25337981]
  13. Cell Mol Life Sci. 2011 Jul;68(13):2303-15 [PMID: 21560068]
  14. J Med Chem. 2013 May 9;56(9):3546-56 [PMID: 23594231]
  15. Front Neurosci. 2017 Feb 14;11:73 [PMID: 28261050]
  16. Biochim Biophys Acta. 2009 Aug;1788(8):1620-9 [PMID: 19059199]
  17. Int J Antimicrob Agents. 2012 Jan;39(1):39-44 [PMID: 22005071]
  18. Mol Immunol. 2010 Jul;47(11-12):2030-7 [PMID: 20451254]
  19. Q Rev Biophys. 1977 Feb;10(1):1-34 [PMID: 327501]
  20. Biochim Biophys Acta. 2008 Feb;1778(2):357-75 [PMID: 18078805]
  21. J Endocrinol. 2006 Jan;188(1):1-9 [PMID: 16394170]
  22. Pharmaceuticals (Basel). 2014 Jan 15;7(1):58-77 [PMID: 24434793]
  23. Molecules. 2019 Aug 16;24(16): [PMID: 31426323]
  24. Peptides. 2006 Jul;27(7):1738-44 [PMID: 16621152]
  25. Biochem Pharmacol. 2017 Jun 1;133:117-138 [PMID: 27663838]
  26. Toxins (Basel). 2018 Oct 13;10(10): [PMID: 30322120]

MeSH Term

Amphibian Proteins
Animals
Anti-Infective Agents
Antineoplastic Agents
Bacteria
Biofilms
Candida albicans
Cell Proliferation
Dose-Response Relationship, Drug
Humans
Kinetics
Neoplasms
PC-3 Cells
Protein Conformation
Ranidae
Structure-Activity Relationship

Chemicals

Amphibian Proteins
Anti-Infective Agents
Antineoplastic Agents
Journal Article

Word Cloud

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