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Diagnostic cytopathology
Nov, 2020;48 (11): 1021-1026.

Contemporary human papillomavirus genotyping and correlations to peniscopy, cytology, and histopatology on over 1000 males.

Jose Carlos Truzzi, Ismael Dale da Silva, Marcos Paulo Freire, Miguel Jorge Neto, Fabio Ferro Rodrigues, Gustavo Arantes Maciel, Ruana Girardi, Sissi Bertolini, Leonardo O Reis
Author Information
  1. Jose Carlos Truzzi: Division of Urology-Fleury Group, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
  2. Ismael Dale da Silva: Division of Molecular Biology, Department of Gynecology-Fleury Group, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
  3. Marcos Paulo Freire: Division of Urology, Universidade Anhembi Morumbi, Sao Paulo, Brazil.
  4. Miguel Jorge Neto: Division of Urology-Fleury Group, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
  5. Fabio Ferro Rodrigues: Division of Urology-Fleury Group, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
  6. Gustavo Arantes Maciel: Division of Molecular Biology-Fleury Group, Universidade de Sao Paulo, Sao Paulo, Brazil.
  7. Ruana Girardi: Division of Urology, Universidade Anhembi Morumbi, Sao Paulo, Brazil.
  8. Sissi Bertolini: Division of Urology, Universidade Anhembi Morumbi, Sao Paulo, Brazil.
  9. Leonardo O Reis: Division of UroScience, University of Campinas and Pontifical Catholic University of Campinas, Sao Paulo, Brazil. ORCID
PMID: 32496006 DOI: 10.1002/dc.24518

Abstract

OBJECTIVES: To explore male human papillomavirus (HPV) contemporary genotyping epidemiology and correlations to peniscopy, cytology, and histopatology.
METHODS: Medical records of patients who had been submitted to HPV infection screening with genotyping, peniscopy, cytology, and histopathology in a period of 2 years were reviewed. Frequency analysis and correlations between the diagnostic tools were established.
RESULTS: Genotype of 1132 men resulted in 69.2% (784) positivity for HPV DNA, 78% classified as high risk of oncogenesis. Co-infections occurred in 429 (54.7%) and the most frequently identified types were HPV-6, HPV-42, and HPV-16, in 133 (17%), 94 (12%), and 86 (11%) patients, respectively. Positive/negative predictive values of peniscopy, cytology, and histopathology were 83/31%, 92/32%, and 87/33%, respectively. As a result, though significant, the correlations between genotype and non-molecular tests were poor.
CONCLUSIONS: In the current contemporary representative male cohort, over two thirds are positive for human HPV DNA, 78% of high risk and with over half co-infections. Though significant, its correlation with non-molecular tests is poor and while the positive predictive values of peniscopy, cytology, and histopatology are between 83% and 92%, their negative predictive values are as low as 31% to 33%.

Keywords

HPV citology diagnosis genotyping histopathology infection men peniscopy

References

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MeSH Term

Adolescent
Adult
Aged
Aged, 80 and over
Alphapapillomavirus
Carcinoma in Situ
Child
Condylomata Acuminata
Cytodiagnosis
DNA, Viral
Genotype
Human papillomavirus 16
Human papillomavirus 6
Humans
Male
Mass Screening
Middle Aged
Papillomavirus Infections
Penile Neoplasms
Penis
Sexual Behavior
Young Adult

Chemicals

DNA, Viral
Journal Article

Word Cloud

Created with Highcharts 10.0.0peniscopyHPVcytologygenotypingcorrelationshumanhistopatologyhistopathologypredictivevaluesmalepapillomaviruscontemporarypatientsinfectionmenDNA78%highriskrespectivelysignificantnon-moleculartestspoorpositiveOBJECTIVES:exploreepidemiologyMETHODS:Medicalrecordssubmittedscreeningperiod2 yearsreviewedFrequencyanalysisdiagnostictoolsestablishedRESULTS:Genotype1132resulted692%784positivityclassifiedoncogenesisCo-infectionsoccurred429547%frequentlyidentifiedtypesHPV-6HPV-42HPV-1613317%9412%8611%Positive/negative83/31%92/32%87/33%resultthoughgenotypeCONCLUSIONS:currentrepresentativecohorttwothirdshalfco-infectionsThoughcorrelation83%92%negativelow31%33%Contemporary1000malescitologydiagnosis

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