Temporal evolution of single-cell transcriptomes of olfactory projection neurons.
Qijing Xie, Maria Brbic, Felix Horns, Sai Saroja Kolluru, Robert C Jones, Jiefu Li, Anay R Reddy, Anthony Xie, Sayeh Kohani, Zhuoran Li, Colleen N McLaughlin, Tongchao Li, Chuanyun Xu, David Vacek, David J Luginbuhl, Jure Leskovec, Stephen R Quake, Liqun Luo, Hongjie Li
Author Information
Qijing Xie: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States. ORCID
Maria Brbic: Department of Computer Science, Stanford University, Stanford, United States.
Felix Horns: Department of Bioengineering, Stanford University, Stanford, United States. ORCID
Sai Saroja Kolluru: Department of Bioengineering, Stanford University, Stanford, United States.
Robert C Jones: Department of Bioengineering, Stanford University, Stanford, United States. ORCID
Jiefu Li: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States. ORCID
Anay R Reddy: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Anthony Xie: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Sayeh Kohani: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Zhuoran Li: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Colleen N McLaughlin: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Tongchao Li: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Chuanyun Xu: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
David Vacek: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
David J Luginbuhl: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Jure Leskovec: Department of Computer Science, Stanford University, Stanford, United States.
Stephen R Quake: Department of Bioengineering, Stanford University, Stanford, United States. ORCID
Liqun Luo: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States. ORCID
Hongjie Li: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Neurons undergo substantial morphological and functional changes during development to form precise synaptic connections and acquire specific physiological properties. What are the underlying transcriptomic bases? Here, we obtained the single-cell transcriptomes of olfactory projection neurons (PNs) at four developmental stages. We decoded the identity of 21 transcriptomic clusters corresponding to 20 PN types and developed methods to match transcriptomic clusters representing the same PN type across development. We discovered that PN transcriptomes reflect unique biological processes unfolding at each stage-neurite growth and pruning during metamorphosis at an early pupal stage; peaked transcriptomic diversity during olfactory circuit assembly at mid-pupal stages; and neuronal signaling in adults. At early developmental stages, PN types with adjacent birth order share similar transcriptomes. Together, our work reveals principles of cellular diversity during brain development and provides a resource for future studies of neural development in PNs and other neuronal types.
