Investigating the action of the microalgal pigment marennine on Vibrio splendidus by in vivoH and P solid-state NMR.
Zeineb Bouhlel, Alexandre A Arnold, Jean-Sébastien Deschênes, Jean-Luc Mouget, Dror E Warschawski, Réjean Tremblay, Isabelle Marcotte
Author Information
Zeineb Bouhlel: Institut des Sciences de la Mer de Rimouski, Université du Québec à Rimouski, G5L 3A1 Rimouski, Canada; Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, H3C 3P8 Montreal, Canada.
Alexandre A Arnold: Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, H3C 3P8 Montreal, Canada.
Jean-Sébastien Deschênes: Mathematics, Computer Science and Engineering Department, Université du Québec à Rimouski, G5L 3A1 Rimouski, Canada.
Jean-Luc Mouget: Mer-Molécules-Santé, MMS, FR CNRS 3473, IUML, Le Mans Université, 72000 Le Mans, France.
Dror E Warschawski: Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, H3C 3P8 Montreal, Canada; Laboratoire des Biomolécules, LBM, CNRS UMR 7203, Sorbonne Université, École normale supérieure, PSL University, 75005 Paris, France.
Réjean Tremblay: Institut des Sciences de la Mer de Rimouski, Université du Québec à Rimouski, G5L 3A1 Rimouski, Canada.
Isabelle Marcotte: Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, H3C 3P8 Montreal, Canada. Electronic address: marcotte.isabelle@uqam.ca.
This work investigates the potential probiotic effect of marennine - a natural pigment produced by the diatom Haslea ostrearia - on Vibrio splendidus. These marine bacteria are often considered a threat for aquaculture; therefore, chemical antibiotics can be required to reduce bacterial outbreaks. In vivoH solid-state NMR was used to probe the effects of marennine on the bacterial membrane in the exponential and stationary phases. Comparisons were made with polymyxin B (PxB) - an antibiotic used in aquaculture and known to interact with Gram(-) bacteria membranes. We also investigated the effect of marennine using P solid-state NMR on model membranes. Our results show that marennine has little effect on phospholipid headgroups dynamics, but reduces the acyl chain fluidity. Our data suggest that the two antimicrobial agents perturb V. splendidus membranes through different mechanisms. While PxB would alter the bacterial outer and inner membranes, marennine would act through a membrane stiffening mechanism, without affecting the bilayer integrity. Our study proposes this microalgal pigment, which is harmless for humans, as a potential treatment against vibriosis.
