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Methods in molecular biology (Clifton, N.J.)
2021;2359 123-130.

Molecularly Imprinted Solid-Phase Extraction Sorbents for the Selective Extraction of Drugs from Human Urine.

Hasan Basan
Author Information
  1. Hasan Basan: Faculty of Pharmacy, Department of Analytical Chemistry, Gazi University, Etiler, Ankara, Turkey. basan@gazi.edu.tr.
PMID: 34410664 DOI: 10.1007/978-1-0716-1629-1_10

Abstract

Development of molecularly imprinted solid-phase extraction (MISPE) sorbents for the removal of drugs from human urine is described. MISPE sorbents are synthesized through free radical polymerization mechanism and non-covalent imprinting approach. After the completion of the polymerization, bulk polymer is ground using an analytical mill and then wet-sieved. Later on, MISPE sorbent has been dry-packed into solid-phase extraction cartridges after the extraction of template drug molecule. Eluates from the cartridges are analyzed using a spectrophotometer or spectrofluorimeter for the determination of target analyte by referring to the calibration curves.

Keywords

Free-radical polymerization Human urine Molecularly imprinted polymers Molecularly imprinted solid-phase extraction Non-covalent imprinting approach Spectrofluorimetry Spectrophotometry Template extraction

References

  1. Basan H, Yarımkaya S (2014) A novel solid-phase extraction-spectrofluorimetric method for the direct determination of atenolol in human urine. Luminescence 29:225–229 [DOI: 10.1002/bio.2532]
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MeSH Term

Chromatography, High Pressure Liquid
Humans
Molecular Imprinting
Pharmaceutical Preparations
Polymers
Solid Phase Extraction
Spectrophotometry

Chemicals

Pharmaceutical Preparations
Polymers
Journal Article

Word Cloud

Created with Highcharts 10.0.0extractionimprintedsolid-phaseMISPEpolymerizationMolecularlysorbentsurineimprintingapproachusingcartridgesExtractionHumanDevelopmentmolecularlyremovaldrugshumandescribedsynthesizedfreeradicalmechanismnon-covalentcompletionbulkpolymergroundanalyticalmillwet-sievedLatersorbentdry-packedtemplatedrugmoleculeEluatesanalyzedspectrophotometerspectrofluorimeterdeterminationtargetanalytereferringcalibrationcurvesImprintedSolid-PhaseSorbentsSelectiveDrugsUrineFree-radicalpolymersNon-covalentSpectrofluorimetrySpectrophotometryTemplate

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