A deep transfer learning framework adapting mixed subdomains is proposed for cross-species plant disease diagnosis. Most existing deep transfer learning studies focus on knowledge transfer between highly correlated domains. These methods may fail to deal with domains that are poorly correlated. In this study, mixed domain images were generated from source and target image groups for improving the correlation between the mixed domain (training dataset) and the target domain (testing dataset). A subdomain alignment mechanism is employed to transfer knowledge from the mixed domain to the target domain. The proposed framework captures the fine-grained information more effectively. Extensive experiments were conducted and prove that the proposed method produces a more effective result compared with existing deep transfer learning technologies for poorly related subdomains.