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Journal of microbiology (Seoul, Korea)
Jan, 2022;60 (1): 128-136.

Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice.

Jaeyeong Park, Eunkyoung Shin, Ji-Hyun Yeom, Younkyung Choi, Minju Joo, Minho Lee, Je Hyeong Kim, Jeehyeon Bae, Kangseok Lee
Author Information
  1. Jaeyeong Park: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
  2. Eunkyoung Shin: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
  3. Ji-Hyun Yeom: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
  4. Younkyung Choi: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
  5. Minju Joo: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
  6. Minho Lee: Department of Microbiology, College of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea.
  7. Je Hyeong Kim: Division of Pulmonology, Department of Internal Medicine, Korea University Ansan Hospital, Ansan, 15355, Republic of Korea.
  8. Jeehyeon Bae: School of Pharmacy, Chung-Ang University, Seoul, 06974, Republic of Korea. jeehyeon@cau.ac.kr.
  9. Kangseok Lee: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea. kangseok@cau.ac.kr.
PMID: 34964948 DOI: 10.1007/s12275-022-1620-3

Abstract

Acinetobacter baumannii causes multidrug resistance, leading to fatal infections in humans. In this study, we showed that Lys AB2 P3-His-a hexahistidine-tagged form of an antimicrobial peptide (AMP) loaded onto DNA aptamer-functionalized gold nanoparticles (AuNP-Apt)-can effectively inhibit A. baumannii infection in mice. When A. baumannii-infected mice were intraperitoneally injected with AuNP-Apt loaded with Lys AB2 P3-His, a marked reduction in A. baumannii colonization was observed in the mouse organs, leading to prominently increased survival time and rate of the mice compared to those of the control mice treated with AuNP-Apt or Lys AB2 P3-His only. This study shows that AMPs loaded onto AuNP-Apt could be an effective therapeutic tool against infections caused by multidrug-resistant pathogenic bacteria in humans.

Keywords

Acinetobacter baumannii antimicrobial peptide gold nanoparticle multidrug-resistant bacteria

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MeSH Term

Acinetobacter Infections
Acinetobacter baumannii
Animals
Anti-Bacterial Agents
Antimicrobial Peptides
Aptamers, Nucleotide
Drug Delivery Systems
Female
Gold
Humans
Metal Nanoparticles
Mice

Chemicals

Anti-Bacterial Agents
Antimicrobial Peptides
Aptamers, Nucleotide
Gold
Evaluation Study Journal Article
Article has an altmetric score of 4

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