Developing a Programmable, Self-Assembling (SLCCNV) Capsid Proteins into "Nanocargo"-like Architecture.
Raja Muthuramalingam Thangavelu, Deepan Sundarajan, Mohammed Riyaz Savaas Umar, Michael Immanuel Jesse Denison, Dharanivasan Gunasekaran, Ganapathy Rajendran, Nallusamy Duraisamy, Krishnan Kathiravan
Author Information
Raja Muthuramalingam Thangavelu: Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India. ORCID
Deepan Sundarajan: Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India.
Mohammed Riyaz Savaas Umar: Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India.
Michael Immanuel Jesse Denison: Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India.
Dharanivasan Gunasekaran: Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India.
Ganapathy Rajendran: Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India.
Nallusamy Duraisamy: Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India.
Krishnan Kathiravan: Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India.
A new era has begun in which pathogens have become useful scaffolds for nanotechnology applications. In this research/study, an attempt has been made to generate an empty cargo-like architecture from a plant pathogenic virus named (SLCCNV). In this approach, SLCCNV coat protein monomers are obtained efficiently by using a yeast expression system. Further, dialysis of purified SLCCNV-CP monomers against various pH modified (5-10) disassembly and assembly buffers produced a self-assembled "Nanocargo"-like architecture, which also exhibited an ability to encapsulate magnetic nanoparticles . Bioinformatics tools were also utilized to predict the possible self-assembly kinetics and bioconjugation sites of coat protein monomers. Significantly, an biocompatibility study using SLCCNV-Nanocargo particles showed low toxicity to the cells, which eventually proved as a potential nanobiomaterial for biomedical applications.
