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Molecular biology reports
Apr, 2022;49 (4): 3247-3258.

Quercetin ameliorates lipopolysaccharide-induced neuroinflammation and oxidative stress in adult zebrafish.

Sukhdev Singh, Kuleshwar Sahu, Lakshay Kapil, Charan Singh, Arti Singh
Author Information
  1. Sukhdev Singh: Department of Pharmacology, ISF College of Pharmacy, Moga, 142001, Punjab, India.
  2. Kuleshwar Sahu: Department of Pharmacology, ISF College of Pharmacy, Moga, 142001, Punjab, India.
  3. Lakshay Kapil: Department of Pharmacology, ISF College of Pharmacy, Moga, 142001, Punjab, India.
  4. Charan Singh: Department of Pharmaceutics, ISF College of Pharmacy, Moga, 142001, Punjab, India.
  5. Arti Singh: Department of Pharmacology, ISF College of Pharmacy, Moga, 142001, Punjab, India. artiniper@gmail.com. ORCID
PMID: 35094209 DOI: 10.1007/s11033-022-07161-2

Abstract

BACKGROUND: Quercetin is a natural flavonoid that is known to have numerous pharmacological activities such as antioxidative, anti-inflammatory, and neuroprotective effects against various neurological disorders. Lipopolysaccharide (LPS) is a potent endotoxin, reported causing several neurological disorders.
AIM: The present study was designed to investigate the possibility that quercetin ameliorates LPS induced oxidative stress and neuroinflammation in adult zebrafish.
MATERIALS AND METHODS: Zebrafish (weighing 470-530 mg) were treated with a single injection of LPS (1 mg/kg) intraperitoneally (i.p.) followed by post-treatment with quercetin (50 and 100 mg/kg; i.p.) for 7 days. After sacrificing brain was harvested and subjected for biochemical, molecular, and histological analyses.
RESULTS: Results revealed post-treatment with quercetin was able to ameliorate the behavioral abnormalities as in novel tank diving test- time spent in the top zone (TSTZ), and the number of entries in the top zone was significantly (p < 0.01) more as compared to time spent in the bottom zone (TSBZ). In the light-dark chamber test- time spent in the light zone (TSLZ), and the number of entries in the light zone were significantly (p < 0.01) more as compared to time spent in the dark compartment (TSDC). Additionally, results of histopathology (H & E stain) studies showed less disruption in neuronal cells as compared to the LPS treated group. Moreover, the results of the molecular analysis revealed that quercetin treatment significantly (p < 0.01) decrease TNF-α and IL-1β levels as compared to LPS treated animals. Further, results of the biochemical analysis reveal that quercetin significantly (p < 0.01) reduces the level of LPO, nitrite, AChEs and increases anti-oxidant GSH.
CONCLUSION: Quercetin treatment helps to prevent oxidative damage and neuroinflammation in LPS treated adult zebrafish.

Keywords

Lipopolysaccharide Neuroinflammation Oxidative stress Quercetin

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MeSH Term

Animals
Inflammation
Lipopolysaccharides
Neuroinflammatory Diseases
Oxidative Stress
Quercetin
Zebrafish

Chemicals

Lipopolysaccharides
Quercetin
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0LPSquercetinzoneQuercetintreatedtimespentsignificantlyp < 001comparedoxidativestressneuroinflammationadultzebrafishresultsneurologicaldisordersLipopolysaccharideamelioratesippost-treatmentbiochemicalmolecularrevealedtest-topnumberentrieslightanalysistreatmentBACKGROUND:naturalflavonoidknownnumerouspharmacologicalactivitiesantioxidativeanti-inflammatoryneuroprotectiveeffectsvariouspotentendotoxinreportedcausingseveralAIM:presentstudydesignedinvestigatepossibilityinducedMATERIALSANDMETHODS:Zebrafishweighing470-530 mgsingleinjection1 mg/kgintraperitoneallyfollowed50100 mg/kg7 dayssacrificingbrainharvestedsubjectedhistologicalanalysesRESULTS:ResultsableamelioratebehavioralabnormalitiesnoveltankdivingTSTZbottomTSBZlight-darkchamberTSLZdarkcompartmentTSDCAdditionallyhistopathologyH&EstainstudiesshowedlessdisruptionneuronalcellsgroupMoreoverdecreaseTNF-αIL-1βlevelsanimalsrevealreduceslevelLPOnitriteAChEsincreasesanti-oxidantGSHCONCLUSION:helpspreventdamagelipopolysaccharide-inducedNeuroinflammationOxidative

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