Far-UVC irradiation (222 nm) is considered an emerging and sustainable solution for future infection and pandemic challenges. We examined the disinfection performance of a krypton-chloride lamp, with a quasi-monochromatic UVC peak at 222 nm, for inactivating airborne microorganisms in a full-scale ventilation duct system. Single-pass disinfection efficacy of far-UVC was determined and compared with that of a conventional mercury-type UVC (254 nm) lamp. Four bacteria, (), (), , and (), as well as bacteriophage P22, were tested under UV exposure with different velocities of duct flows. The data revealed that as the air velocity increased from 0.7 to 4 m/s, the far-UVC disinfection efficacies would decrease by 42, 47, 35, 39, and 33% for these five microorganisms, respectively. The inactivation rate constants to far-UVC light were 4.9, 7.5, 3.3, 6.3, and 3.0 cm/mJ for aerosolized , , , , and bacteriophage P22, respectively. Far-UVC irradiation showed a comparable disinfection ability on airborne microorganisms compared with the 254 nm UV irradiation. This first study of far-UVC in real duct applications provides a better understanding of the disinfection performance of this solution in bioaerosol inactivation. It offers a valuable database in the sizing and design of excimer lamps for novel portable air purifiers or in-duct disinfection units.