A highquality genome assembly and annotation of Carruth.
Dan Liu, Xiaoman Xie, Boqiang Tong, Chengcheng Zhou, Kai Qu, Haili Guo, Zhiheng Zhao, Yousry A El-Kassaby, Wei Li, Wenqing Li
Author Information
Dan Liu: National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Xiaoman Xie: Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan, China.
Boqiang Tong: Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan, China.
Chengcheng Zhou: National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Kai Qu: National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Haili Guo: Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan, China.
Zhiheng Zhao: National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Yousry A El-Kassaby: Department of Forest and Conservation Sciences, The University of British Columbia, Vancouver, BC, Canada.
Wei Li: National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Wenqing Li: Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan, China.
Introduction: is an economic and ecological tree species often used for afforestation of arid and semi-arid lands and is considered as an excellent tree for soil and water conservation. Methods: Here, we combined PacBio long reads, Hi-C, and Illumina short reads to assemble genome. Results: We generated a 957.1 Mb genome with a contig N50 of 1.2 Mb and scaffold N50 of 77.0 Mb. The repetitive sequences constituted 55.63% of the genome, among which long terminal repeats were the majority and accounted for 23.07% of the genome. , homology-based and RNA sequence-based gene prediction identified 29,889 protein-coding genes, of which 82.6% could be functionally annotated. Phylogenetic analysis showed that and were differentiated around 3.6 million years ago, and showed no evidence of species-specific whole genome duplication. Conclusion: The assembled and annotated high-quality genome not only promises to accelerate the species molecular biology studies and breeding, but also promotes genome level evolutionary studies.
