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Bioelectromagnetics
Apr, 2024;45 (3): 97-109.

The effect of exposure to radiofrequency LTE signal and coexposure to mitomycin-C in Chinese hamster lung fibroblast V79 cells.

Anna Sannino, Stefania Romeo, Maria Rosaria Scarfì, Daniele Pinchera, Fulvio Schettino, Mario Alonzo, Mariateresa Allocca, Olga Zeni
Author Information
  1. Anna Sannino: National Research Council of Italy (CNR), Institute for Electromagnetic Sensing of the Environment (IREA), Naples, Italy. ORCID
  2. Stefania Romeo: National Research Council of Italy (CNR), Institute for Electromagnetic Sensing of the Environment (IREA), Naples, Italy. ORCID
  3. Maria Rosaria Scarfì: National Research Council of Italy (CNR), Institute for Electromagnetic Sensing of the Environment (IREA), Naples, Italy. ORCID
  4. Daniele Pinchera: Department of Electrical and Information Engineering "Maurizio Scarano" (DIEI), University of Cassino and Southern Lazio, Cassino, Italy. ORCID
  5. Fulvio Schettino: Department of Electrical and Information Engineering "Maurizio Scarano" (DIEI), University of Cassino and Southern Lazio, Cassino, Italy. ORCID
  6. Mario Alonzo: National Research Council of Italy (CNR), Institute for Electromagnetic Sensing of the Environment (IREA), Naples, Italy. ORCID
  7. Mariateresa Allocca: National Research Council of Italy (CNR), Institute for Electromagnetic Sensing of the Environment (IREA), Naples, Italy. ORCID
  8. Olga Zeni: National Research Council of Italy (CNR), Institute for Electromagnetic Sensing of the Environment (IREA), Naples, Italy. ORCID
PMID: 37493434 DOI: 10.1002/bem.22478

Abstract

This study aims to investigate the cellular effects of radiofrequency exposure, 1950 MHz, long-term evolution (LTE) signal, administered alone and in combination with mitomycin-C (MMC), a well-known cytotoxic agent. Chinese hamster lung fibroblast (V79) cells were exposed/sham exposed in a waveguide-based system under strictly controlled conditions of both electromagnetic and environmental parameters, at specific absorption rate (SAR) of 0.3 and 1.25 W/kg. Chromosomal damage (micronuclei formation), oxidative stress (reactive oxygen species [ROS] formation), and cell cycle progression were analyzed after exposure and coexposure. No differences between exposed samples and sham-controls were detected following radiofrequency exposure alone, for all the experimental conditions tested and biological endpoints investigated. When radiofrequency exposure was followed by MMC treatment, 3 h pre-exposure did not modify MMC-induced micronuclei. Pre-exposure of 20 h at 0.3 W/kg did not modify the number of micronuclei induced by MMC, while 1.25 W/kg resulted in a significant reduction of MMC-induced damage. Absence of effects was also detected when CW was used, at both SAR levels. MMC-induced ROS formation resulted significantly decreased at both SAR levels investigated, while cell proliferation and cell cycle progression were not affected by coexposures. The results here reported provide no evidence of direct effects of 1950 MHz, LTE signal. Moreover, they further support our previous findings on the capability of radiofrequency pre-exposure to induce protection from a subsequent toxic treatment, and the key role of the modulated signals and the experimental conditions adopted in eliciting the effect.

Keywords

LTE coexposure in vitro radiofrequency

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Grants

  1. Grant 2017SAKZ78 - PRIN Project MIRABILIS/Ministero dell'Università e della Ricerca

MeSH Term

Cricetinae
Animals
Cricetulus
Mitomycin
Reactive Oxygen Species
Lung
Fibroblasts

Chemicals

Mitomycin
Reactive Oxygen Species
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0radiofrequencyexposureLTEeffectssignalMMCconditionsSARmicronucleiformationcellcoexposureMMC-induced1950 MHzalonemitomycin-CChinesehamsterlungfibroblastV79cellsexposed0125 W/kgdamagecycleprogressiondetectedexperimentalinvestigatedtreatmentpre-exposuremodifyresultedlevelseffectstudyaimsinvestigatecellularlong-termevolutionadministeredcombinationwell-knowncytotoxicagentexposed/shamwaveguide-basedsystemstrictlycontrolledelectromagneticenvironmentalparametersspecificabsorptionrate3Chromosomaloxidativestressreactiveoxygenspecies[ROS]analyzeddifferencessamplessham-controlsfollowingtestedbiologicalendpointsfollowed3 hPre-exposure20 h3 W/kgnumberinducedsignificantreductionAbsencealsoCWusedROSsignificantlydecreasedproliferationaffectedcoexposuresresultsreportedprovideevidencedirectMoreoversupportpreviousfindingscapabilityinduceprotectionsubsequenttoxickeyrolemodulatedsignalsadoptedelicitingvitro

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