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Nanoscale advances
Sep 12, 2023;5 (18): 4681-4686.

A graphene microelectrode array based microfluidic device for continuous monitoring of biofilms.

Jin Song, Ashaq Ali, Yaohong Ma, Yiwei Li
Author Information
  1. Jin Song: Biology Institute, Qilu University of Technology (Shandong Academy of Sciences) Jinan 250103 China.
  2. Ashaq Ali: Center of Excellence in Science & Applied Technologies (CESAT) Islamabad 75000 Pakistan.
  3. Yaohong Ma: Biology Institute, Qilu University of Technology (Shandong Academy of Sciences) Jinan 250103 China.
  4. Yiwei Li: Biology Institute, Qilu University of Technology (Shandong Academy of Sciences) Jinan 250103 China. ORCID
PMID: 37705780 DOI: 10.1039/d3na00482a

Abstract

continuous monitoring of bacterial biofilms has been a challenging job so far, but it is fundamental to the screening of novel anti-biofilm reagents. In this work, a microfluidic system utilizing a graphene-modified microelectrode array sensor was proposed to realize the dynamic state of bacterial biofilm monitoring by electrochemical impedance. The results illustrated that the observation window period of the biofilm state is significantly prolonged due to the increment of bacterial cell load on the sensing interface, thereby greatly improving the sensing signal quality. Simulation of anti-biofilm drug screening demonstrated that the performance of this method manifestly exceeded that of its endpoint counterparts.

References

  1. Clin Oral Investig. 2014;18(2):625-34 [PMID: 23716063]
  2. ACS Sens. 2020 Jun 26;5(6):1795-1803 [PMID: 32397709]
  3. Mol Microbiol. 1998 May;28(3):449-61 [PMID: 9632250]
  4. Nat Commun. 2014 Oct 20;5:5259 [PMID: 25327632]
  5. Molecules. 2015 Mar 24;20(4):5286-98 [PMID: 25812150]
  6. Sci Total Environ. 2021 Apr 15;765:144264 [PMID: 33418325]
  7. PLoS One. 2020 Sep 17;15(9):e0238428 [PMID: 32941438]
  8. Sens Actuators B Chem. 2023 Feb 1;376(Pt A): [PMID: 36688105]
  9. J Clin Microbiol. 2016 Mar;54(3):657-61 [PMID: 26719437]
  10. Nat Rev Microbiol. 2007 Jan;5(1):48-56 [PMID: 17143318]
  11. J Chin Med Assoc. 2018 Jan;81(1):7-11 [PMID: 29042186]
  12. Int J Mol Sci. 2023 Jan 05;24(2): [PMID: 36674518]
  13. Viruses. 2020 Apr 07;12(4): [PMID: 32272740]
  14. Exp Biol Med (Maywood). 2020 Jan;245(1):34-41 [PMID: 31903777]
  15. Lab Chip. 2007 Dec;7(12):1723-31 [PMID: 18030393]
  16. J Biomed Mater Res B Appl Biomater. 2022 May;110(5):1044-1055 [PMID: 34897974]
  17. Clin Microbiol Rev. 2011 Jan;24(1):29-70 [PMID: 21233507]
  18. Microbiologyopen. 2019 Apr;8(4):e00695 [PMID: 30051653]
  19. Water Res. 2022 May 15;215:118233 [PMID: 35248909]
  20. Nat Protoc. 2010 Aug;5(8):1460-9 [PMID: 20671729]
  21. Nat Protoc. 2010 Jul;5(7):1236-54 [PMID: 20595953]
  22. Biomed Mater. 2022 Feb 14;17(2): [PMID: 35105823]
  23. Nat Rev Microbiol. 2017 Aug;15(8):453-464 [PMID: 28529326]
  24. Biosens Bioelectron. 2020 Feb 1;149:111874 [PMID: 31780407]
  25. J Med Microbiol. 2021 May;70(5): [PMID: 34048334]
  26. Microsyst Nanoeng. 2020 Oct 19;6:81 [PMID: 34567691]
  27. Heliyon. 2018 Dec 28;4(12):e01067 [PMID: 30619958]
  28. Chemosphere. 2022 May;294:133609 [PMID: 35051518]
  29. ACS Nano. 2019 May 28;13(5):4869-4875 [PMID: 31033283]
  30. Antimicrob Resist Infect Control. 2019 May 16;8:76 [PMID: 31131107]
  31. Front Cell Infect Microbiol. 2022 Jan 05;11:795797 [PMID: 35071046]
Journal Article

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