Use of Galleria mellonella as an Animal Model for Studying the Antimicrobial Activity of Bacteriophages with Potential Use in Phage Therapy.

Lucía Blasco, María Tomás
Author Information
  1. Lucía Blasco: Traslational and Multidisciplinary Microbiology (MicroTM), Biomedical Research Institute A Coruña (INIBIC), Microbiology Department, Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain.
  2. María Tomás: Traslational and Multidisciplinary Microbiology (MicroTM), Biomedical Research Institute A Coruña (INIBIC), Microbiology Department, Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain. MA.del.Mar.Tomas.Carmona@sergas.es.

Abstract

Interest in phage therapy has increased in the last decade, and animal models have become essential in this field. The larval stage of the wax moth, Galleria mellonella, represents an easy-to-handle model. The larvae have an innate immune response and survive at 37 °C, which is ideal for infection and antimicrobial studies with bacteriophages. In this chapter, we describe the procedures used to study the antimicrobial activity of bacteriophages in a G. mellonella infection model.

Keywords

References

  1. Andrea A, Krogfelt KA, Jenssen H (2019) Methods and challenges of using the greater wax moth (Galleria mellonella) as a model organism in antimicrobial compound discovery. Microorganisms 7(3):85 [DOI: 10.3390/microorganisms7030085]
  2. Harding CR, Schroeder GN, Collins JW et al (2013) Use of galleria mellonella as a model organism to study Legionella pneumophila infection. J Vis Exp 2013(81):e50964
  3. Mukherjee K, Domann E, Hain T (2011) The greater wax moth Galleria mellonella as an alternative model host for human pathogens. In: Vilcinskas A (ed) Insect biotechnology. Springer Netherlands, Dordrecht, pp 3–14 [DOI: 10.1007/978-90-481-9641-8_1]
  4. Jander G, Rahme LG, Ausubel FM (2000) Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects. J Bacteriol 182(13):3843–3845 [DOI: 10.1128/JB.182.13.3843-3845.2000]
  5. Kavanagh K, Reeves EP (2004) Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens. FEMS Microbiol Rev 28(1):101–112 [DOI: 10.1016/j.femsre.2003.09.002]
  6. Tsai CJ, Loh JM, Proft T (2016) Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing. Virulence 7(3):214–229 [DOI: 10.1080/21505594.2015.1135289]
  7. Seed KD, Dennis JJ (2009) Experimental bacteriophage therapy increases survival of Galleria mellonella larvae infected with clinically relevant strains of the burkholderia cepacia complex. Antimicrob Agents Chemother 53(5):2205–2208 [DOI: 10.1128/AAC.01166-08]
  8. El Haddad L, Angelidakis G, Clark JR et al (2022) Genomic and functional characterization of vancomycin-resistant Enterococci-specific bacteriophages in the Galleria mellonella wax moth larvae model. Pharmaceutics 14(8):1591 [DOI: 10.3390/pharmaceutics14081591]
  9. Tkhilaishvili T, Wang L, Tavanti A et al (2020) Antibacterial efficacy of two commercially available bacteriophage formulations, staphylococcal bacteriophage and pyo bacteriophage, against methicillin-resistant staphylococcus aureus: prevention and eradication of biofilm formation and control of a systemic infection of Galleria mellonella larvae. Front Microbiol 11:110 [DOI: 10.3389/fmicb.2020.00110]
  10. Kazmierczak N, Grygorcewicz B, Roszak M et al (2022) Comparative assessment of bacteriophage and antibiotic activity against multidrug-resistant Staphylococcus aureus biofilms. Int J Mol Sci 23(3):1274 [DOI: 10.3390/ijms23031274]
  11. Blasco L, Ambroa A, Lopez M et al (2019) Combined use of the ab105-2phideltaci lytic mutant phage and different antibiotics in clinical isolates of multi-resistant Acinetobacter baumannii. Microorganisms 7(11):556 [DOI: 10.3390/microorganisms7110556]
  12. Pu M, Han P, Zhang G et al (2022) Characterization and comparative genomics analysis of a new bacteriophage buct610 against Klebsiella pneumoniae and efficacy assessment in galleria mellonella larvae. Int J Mol Sci 23(14):8040 [DOI: 10.3390/ijms23148040]
  13. Jeon J, Park JH, Yong D (2019) Efficacy of bacteriophage treatment against carbapenem-resistant Acinetobacter baumannii in Galleria mellonella larvae and a mouse model of acute pneumonia. BMC Microbiol 19(1):70 [DOI: 10.1186/s12866-019-1443-5]
  14. Jeon J, Yong D (2019) Two novel bacteriophages improve survival in galleria mellonella infection and mouse acute pneumonia models infected with extensively drug-resistant Pseudomonas aeruginosa. Appl Environ Microbiol 85(9):e02900–e02918 [DOI: 10.1128/AEM.02900-18]
  15. Manohar P, Nachimuthu R, Lopes BS (2018) The therapeutic potential of bacteriophages targeting gram-negative bacteria using Galleria mellonella infection model. BMC Microbiol 18(1):97 [DOI: 10.1186/s12866-018-1234-4]
  16. Blasco L, Ambroa A, Trastoy R et al (2020) In vitro and in vivo efficacy of combinations of colistin and different endolysins against clinical strains of multi-drug resistant pathogens. Sci Rep 10(1):7163 [DOI: 10.1038/s41598-020-64145-7]
  17. Chen X, Liu M, Zhang P et al (2022) Phage-derived depolymerase as an antibiotic adjuvant against multidrug-resistant Acinetobacter baumannii. Front Microbiol 13:845500 [DOI: 10.3389/fmicb.2022.845500]
  18. Kropinski AM, Mazzocco A, Waddell TE et al (2009) Enumeration of bacteriophages by double agar overlay plaque assay. Methods Mol Biol 501:69–76 [DOI: 10.1007/978-1-60327-164-6_7]
  19. Kutter E (2009) Phage host range and efficiency of plating. Methods Mol Biol 501:141–149 [DOI: 10.1007/978-1-60327-164-6_14]
  20. Kropinski AM (2018) Practical advice on the one-step growth curve. Methods Mol Biol 1681:41–47 [DOI: 10.1007/978-1-4939-7343-9_3]

MeSH Term

Animals
Bacteriophages
Phage Therapy
Disease Models, Animal
Moths
Larva

Word Cloud

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