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Electrophoresis
Apr, 2024;45 (7-8): 618-638.

Mobilization in two-step capillary isoelectric focusing: Concepts assessed by computer simulation.

Wolfgang Thormann, Richard A Mosher
Author Information
  1. Wolfgang Thormann: Institute for Infectious Diseases, University of Bern, Bern, Switzerland. ORCID
  2. Richard A Mosher: RAM Software Solutions, Tucson, Arizona, USA.
PMID: 38115749 DOI: 10.1002/elps.202300218

Abstract

The mobilization step in a two-step capillary isoelectric focusing protocol is discussed by means of dynamic computer simulation data for systems without and with spacer compounds that establish their zones at the beginning and end of the focusing column. After focusing in an electroosmosis-free environment (first step), mobilization (second step) can be induced electrophoretically, by the application of a hydrodynamic flow, or by a combination of both means. Dynamic simulations provide insight into the complexity of the various modes of electrophoretic mobilization and dispersion associated with hydrodynamic mobilization. The data are discussed together with the relevant literature.

Keywords

capillary isoelectric focusing chemical mobilization computer simulation electrophoretic mobilization hydrodynamic mobilization

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MeSH Term

Capillary Isoelectric Focusing
Computer Simulation
Electrophoresis, Capillary
Hydrodynamics
Journal Article Research Support, Non-U.S. Gov't Review

Word Cloud

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