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Cell structure and function
Nov 07, 2024;49 (2): 101-110.

Cell-wide arrangement of Golgi/RE units depends on the microtubule organization.

Tatsuya Tago, Syara Fujii, Shogo Sasaki, Maki Shirae-Kurabayashi, Naoaki Sakamoto, Takashi Yamamoto, Makoto Maeda, Tatsuya Ueki, Takunori Satoh, Akiko K Satoh
Author Information
  1. Tatsuya Tago: Program of Life and Environmental Science, Graduate School of Integral Science for Life, Hiroshima University.
  2. Syara Fujii: Program of Life and Environmental Science, Graduate School of Integral Science for Life, Hiroshima University.
  3. Shogo Sasaki: Program of Life and Environmental Science, Graduate School of Integral Science for Life, Hiroshima University.
  4. Maki Shirae-Kurabayashi: Sugashima Marine Biological Laboratory, Graduate School of Science, Nagoya University.
  5. Naoaki Sakamoto: Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University.
  6. Takashi Yamamoto: Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University.
  7. Makoto Maeda: Natural Science Center for Basic Research and Development, Hiroshima University.
  8. Tatsuya Ueki: Program of Basic Biology, Graduate School of Integrated Sciences for Life, Hiroshima University.
  9. Takunori Satoh: Program of Life and Environmental Science, Graduate School of Integral Science for Life, Hiroshima University.
  10. Akiko K Satoh: Program of Life and Environmental Science, Graduate School of Integral Science for Life, Hiroshima University.
PMID: 39358226 DOI: 10.1247/csf.24055

Abstract

We have previously shown that Golgi stacks and recycling endosomes (REs) exist as Golgi/RE units in sea urchin embryos. In this study, we showed that Golgi/RE units were scattered throughout the cytoplasm at early developmental stages but gathered to form a "Golgi ring" surrounding the centric REs at the blastula stage. This change in the cell-wide arrangement of Golgi/RE units coincided with a dramatic change in microtubule organization from a randomly oriented cortical pattern to radial arrays under the apical plasma membrane. A single gigantic Golgi apparatus surrounding centric RE is clearly associated with the center of the radial microtubule arrays. Furthermore, we found that in some animal species belonging to different clades, Golgi stacks lack lateral connections but are likely centralized by microtubule motors. These results suggest that Golgi centralization depends on the organization of the microtubule array in addition to the lateral linking between Golgi stacks.Key words: Golgi stack, recycling endosome, Golgi-ribbon, microtubule, cilium, sea urchin, ascidian.

Keywords

Golgi stack Golgi-ribbon ascidian cilium microtubule recycling endosome sea urchin

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MeSH Term

Golgi Apparatus
Microtubules
Animals
Endosomes
Sea Urchins
Journal Article
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Word Cloud

Created with Highcharts 10.0.0GolgimicrotubuleGolgi/REunitsstacksrecyclingseaurchinorganizationREssurroundingcentricchangearrangementradialarrayslateraldependsstackendosomeGolgi-ribbonciliumascidianpreviouslyshownendosomesexistembryosstudyshowedscatteredthroughoutcytoplasmearlydevelopmentalstagesgatheredform"Golgiring"blastulastagecell-widecoincideddramaticrandomlyorientedcorticalpatternapicalplasmamembranesinglegiganticapparatusREclearlyassociatedcenterFurthermorefoundanimalspeciesbelongingdifferentcladeslackconnectionslikelycentralizedmotorsresultssuggestcentralizationarrayadditionlinkingKeywords:Cell-wide

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