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Nutrition (Burbank, Los Angeles County, Calif.)
Nov 29, 2024;131 112656.

Effects of feeding the medium-chain triacylglycerol on GLP-1 secretion in aged Brd4-heterozygous mice, which are a mouse model of aging.

Musashi Kawamura, Natsuyo Hariya, Shiori Ishiyama, Yuji Tanaka, Keiko Ozato, Kazuki Mochizuki
Author Information
  1. Musashi Kawamura: Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi, Japan; Department of Local Produce and Food Sciences, Laboratory of Food and Nutritional Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.
  2. Natsuyo Hariya: Department of Nutrition and Dietetics, Faculty of Family and Consumer Sciences, Kamakura Women's University, Kamakura, Kanagawa, Japan.
  3. Shiori Ishiyama: Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi, Japan; Department of Local Produce and Food Sciences, Laboratory of Food and Nutritional Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.
  4. Yuji Tanaka: New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan.
  5. Keiko Ozato: Laboratory of Molecular Growth Regulation, NICHD, NIH, Bethesda, Maryland, USA.
  6. Kazuki Mochizuki: Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi, Japan; Department of Local Produce and Food Sciences, Laboratory of Food and Nutritional Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi, Japan. Electronic address: mochizukik@yamanashi.ac.jp.
PMID: 39708424 DOI: 10.1016/j.nut.2024.112656

Abstract

OBJECTIVES: Gastrointestinal hormones, such as glucagon-like peptide 1 (GLP-1), gastric inhibitory polypeptide, and peptide YY (PYY) are important for reducing malnutrition at older ages because they are related to assimilation and feeding behavior. Medium-chain triacylglycerol (MCT) ameliorates metabolic symptoms and frailty in adults; however, whether it has the same effect in old age is unknown. To address this, we examined the changes in insulin and gastrointestinal hormones in aged Brd4 (+/-) mice exhibiting symptoms of old age.
RESEARCH METHODS AND PROCEDURES: Aged male wild-type and Brd4 (+/-) mice were fed a long-chain triacylglycerol (LCT)- or MCT diet. Feeding, blood glucose, and plasma active GLP-1 protein concentrations were determined at 9 weeks using a meal tolerance test, and those gastrointestinal hormone genes were determined at 10 weeks.
RESULTS: The liver and stomach weights and mRNA expression of Gcg (encodes GLP-1 protein) and Pyy in the colon were lower in LCT-fed Brd4 (+/-) mice than those in LCT-fed wild-type mice; these were restored by the MCT diet. The blood concentration of active GLP-1 protein at 15 and 30 minutes postload was higher in MCT-fed Brd4 (+/-) mice than that in those fed an LCT diet.
CONCLUSIONS: Aged Brd4 (+/-) mice showed lower mRNA expression of Gcg and Pyy genes, and active GLP-1 protein secretion in the blood, which were as restored and enhanced with MCT feeding.

Keywords

Aging GLP-1 Gastrointestinal hormones Gcg Insulin Pyy
Journal Article

Word Cloud

Created with Highcharts 10.0.0GLP-1miceBrd4+/-MCTproteinhormonesfeedingtriacylglyceroldietbloodactiveGcgPyyGastrointestinalpeptidesymptomsoldagegastrointestinalagedAgedwild-typefedLCTdeterminedweeksgenesmRNAexpressionlowerLCT-fedrestoredsecretionOBJECTIVES:glucagon-like1gastricinhibitorypolypeptideYYPYYimportantreducingmalnutritionolderagesrelatedassimilationbehaviorMedium-chainamelioratesmetabolicfrailtyadultshoweverwhethereffectunknownaddressexaminedchangesinsulinexhibitingRESEARCHMETHODSANDPROCEDURES:malelong-chain-Feedingglucoseplasmaconcentrations9usingmealtolerancetesthormone10RESULTS:liverstomachweightsencodescolonconcentration1530minutespostloadhigherMCT-fedCONCLUSIONS:showedenhancedEffectsmedium-chainBrd4-heterozygousmousemodelagingAgingInsulin

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